- Title
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Expression of the G protein gammaT1 subunit during zebrafish development
- Authors
- Chen, H., Leung, T., Giger, K.E., Stauffer, A.M., Humbert, J.E., Sinha, S., Horstick, E.J., Hansen, C.A., and Robishaw, J.D.
- Source
- Full text @ Gene Expr. Patterns
Tissue distribution of gngT1. RT-PCR analysis was performed on various tissues from 6-month old zebrafish. PCR amplification was carried out for the indicated number of cycles for gngT1 and β-actin. The latter one was used as a loading control. |
Expression pattern of gngT1 during zebrafish development. (a) Lateral view and (b) dorsal view of whole mount in situ hybridization of gngT1 in the zebrafish embryos from 24 to 60 hpf. (c) Two-color in situ hybridization using aanat-2 and gngT1 probes at night (44 hpf). Fast red staining for aanat-2 (red) and NBT/BCIP for gngT1 (blue). Flu, fluorescein in red; Dig, digoxigenin in blue. Arrow points to the pineal and arrowheads point to the eyes. |
Comparison of the gngT1 and exorh expression in the pineal gland. (a) Lateral view of the gngT1 and exorh transcripts at 24, 48 and 72 hpf. (b) Two-color in situ hybridization using exorh and gngT1 probes at 48 hpf. Fast red staining for exorh (red) and NBT/BCIP for gngT1 (blue). exorh, exo-rhodopsin; Flu, fluorescein; Dig, digoxigenin. EXPRESSION / LABELING:
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Comparison of the gngT1 and rho expression in the retina. (a) Lateral view of the gngT1 and rhodopsin transcripts at 60, 72 and 84 hpf. (b) Two-color in situ hybridization using gngT1 and rhodopsin probes in the retinal photoreceptor layer at 72 hpf. Fast red staining for rho (red) and NBT/BCIP for gngT1 (blue). rho, rhodopsin; Flu, fluorescein; Dig, digoxigenin. Scale bar equals 50μm. EXPRESSION / LABELING:
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Regulation of the zebrafish gngT1 expression by otx5 in the pineal gland. The embryos were injected with MO-otx5 and the transcripts were detected by Dig labeled gngT1 probe. (a) Dorsal view of gngT1 expression in the pineal gland. (b) Lateral view of gngT1 expression in the pineal and the retina at 72 hpf. (c) Expression of the GFP in the pineal gland at 72 hpf using the transgenic zebrafish line TG[aanat-2:GFP]. EXPRESSION / LABELING:
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Regulation of zebrafish gngT1 expression by crx in the retina. The embryos were injected with MO-crx and the transcripts were detected by Dig labeled gngT1 probe. (a) Dorsal view of gngT1 expression in the pineal gland at 48 hpf. (b) Lateral view of gngT1 expression in the pineal gland and the retina at 72 hpf. (c) Plastic section of zebrafish eyes at 72 hpf. EXPRESSION / LABELING:
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Reprinted from Gene expression patterns : GEP, 7(5), Chen, H., Leung, T., Giger, K.E., Stauffer, A.M., Humbert, J.E., Sinha, S., Horstick, E.J., Hansen, C.A., and Robishaw, J.D., Expression of the G protein gammaT1 subunit during zebrafish development, 574-583, Copyright (2007) with permission from Elsevier. Full text @ Gene Expr. Patterns