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Thisse et al., 2005 - High Throughput Expression Analysis of ZF-Models Consortium Clones. ZFIN Direct Data Submission
Submitted By: Fauny, Jean-Daniel, Hamadbachir, Amira, Hartmeyer, Aline, Thisse, Bernard, Thisse, Christine (Citing this work)
In situ hybridizations for this high throughput analysis have been performed once. Mistakes may occur. Please contact C and B Thisse if you detect anything wrong.
Thisse in situ hybridization protocol
Probe: eu580   Quality: Rating 5

Fig. 1 no expression

EXPRESSION / LABELING:
Gene:
Fish:
Stage Range: 50%-epiboly to Bud

Fig. 2 very weak in Kupffer's vesicle, adaxial cells, 2 rhombomeres

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 10-13 somites

Fig. 3 dorsal CNS from MHB to tail, cranial ganglia, heart primordium, somites

Fig. 4 olfactory placodes, lens, nuclei in ventral posterior midbrain, epiphysis, MHB, dorsal hindbrain +++, cranial ganglia, ventral hindbrain, heart, myotomes, spinal chord neurons

Fig. 5 nose, epiphysis, ventral posterior tegmentum, dorsal cerebellum, dorsal hindbrain, cranial ganglia, nuclei in ventral hindbrain, ventral part of retina, habenula (left, very weak expression on right side), hypaxial part of anterior somites, spinal chord neurons.

Fig. 6 nose, epiphysis, ventral posterior tegmentum, dorsal cerebellum, dorsal hindbrain, cranial ganglia, nuclei in ventral hindbrain, ventral part of retina, habenula (left, very weak expression on right side), hypaxial part of anterior somites, spinal chord neurons, retina intermediate cell layer, pectoral fin muscles, hypaxial muscles

Fig. 7 habenula (more in left side but asymmetry not very strong at that stage).
Please note that in 5 day old embryos some structures are not accessible to the probe (such as notochord, most of the trunk and tail). Therefore the description of the expression pattern is only partial.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 5