Sun et al., 2004 - A Genetic Screen in Zebrafish Identifies Cilia Genes as a Principal Cause of Cystic Kidney Development. Development (Cambridge, England)   131(16):4085-4093 Full text @ Development

Fig. 1

Phenotypes of identified mutants. (A) A larryhi409 mutant on day 5 shows kidney cyst (arrow) and body curvature. (B,C) Cross-section of the glomerular (arrow)-tubular (arrowhead) region of wild-type (B) and larryhi409 (C) embryos at 50 hpf (hour post fertilization). (D) pkd2 morphant at 53 hpf shows kidney cyst (arrow) and body curvature. nc, notochord; wt, wild type; mut, mutant; control, embryo injected with standard control morpholino oligo; mph, morphant.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 3

hi409/IFT81 is widely expressed. (A) In situ hybridization for hi409/IFT81 in an embryo at the eight-somite stage (8S), hi409/IFT81 is enriched in the notochord (arrow). (B) In embryos at 25 hpf, hi409/IFT81 is enriched in the brain ventricle (arrow). (C) In embryos at 34 hpf, hi409/IFT81 is enriched in the otic vesicle (arrow) and slightly in the pronephric duct (arrowhead).

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15

Fig. 4

Ciliary defects in scorpionhi459. (A) The pronephric duct region (arrow) in a wild-type embryo at 50 hpf reacts strongly with an antibody against acetylated tubulin. (B) Confocal image shows cilia stained with this antibody in the pronephric duct of a wild-type embryo at 24 hpf. (D) Defect of the cilia in scorpionhi459 at 50 hpf; (D) enlarged view of C. wt, wild type; 459, scorpionhi459.

Fig. 5

Pronephric ducts. (A,B) Cross-section through pronephric ducts (arrow on the right side; encircled on the left) in wild-type (wt) and larryhi409 mutant (409) embryos. (C,D) Dorsal view of the pronephric ducts stained with α6F. nc, notochord.

PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Table 2 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development