Melby et al., 2000 - Patterning the early zebrafish by the opposing actions of bozozok and vox/vent. Developmental Biology   224(2):275-285 Full text @ Dev. Biol.

Fig. 2 Expression of vox and vent during development. (A, B) 30% epiboly, animal pole view. (C, D) Shield stage, animal pole view. Arrowheads mark the dorsal border of vox (C) and vent (D) expression. (E, F) Shield stage, dorsal view. (G, H) Bud stage, posterior view. (I, J) 22-somite embryos, lateral view. (K) 70% epiboly, dorsal view, anterior is at the top. Note the clearing of vox from the axial region (asterisk). (L) 5- to 7-somite stage, dorsal view of embryo dissected away from the yolk, anterior to the top. Arrows mark expression in the neural crest. (M) 12-somite embryo, side view, anterior to the top. Arrowhead marks expression in the eye.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 20-25 somites

Fig. 3 Expression of vox and vent in dorsalized zebrafish mutants. (A–F) Expression of vox and vent in 70% epiboly embryos, lateral view, dorsal is to the right. (A, B) Wild-type (wt) embryos. (C, D) swirl (swr) mutants. (E, F) somitabun (sbn) mutants. Note that the marginal expression of vox is retained in swr (C) and sbn (E) embryos. (G–J) Expression of vox and vent in 100% epiboly embryos, vegetal view, dorsal is to the top. (G, H) Wild-type embryos. (I, J) snailhouse (snh) embryos. Note that the expression level is reduced in snh embryos but the pattern of expression is unchanged.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Bud
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to Bud

Fig. 4 Ventralization of embryos by ectopic vent expression. (A) Wild-type embryo. (B) vent-injected embryo, V2 phenotype. (C) vent-injected embryo, V3 phenotype. Embryos in the V2 class lack head structures such as eyes and hatching gland and have a partial loss of notochord. Embryos in the more strongly ventralized V3 class have a complete lack of forebrain and notochord.

Fig. 6 Inhibition of boz expression by vox and vent. (A) Untreated embryo, dorsal to the right. boz is expressed in a small group of dorsal cells and the dorsal YSL. (B) LiCl-treated embryo. boz expression is expanded throughout the marginal blastomeres and YSL. (C, D) vox (C) or vent (D) RNA-injected embryos treated with LiCl. Most of the boz expression has been eliminated in these embryos. In some embryos, expression of boz was completely eliminated. Embryos are at sphere stage. Side views with dorsal to the right.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Sphere

Fig. 7 bozozok regulates the expression of vox and vent. (A–D) Expression of vox (A, B) and vent (B, D) in wild-type (+; A, C) and boz mutant (B, D) embryos at 30% epiboly. Note that vox and vent are expressed in the dorsal region in boz mutants. (E–H) Expression of vox (E, F) and vent (G, H) in uninjected (control; E, G) and boz-injected (G, H) embryos at shield stage. Dorsal is to the right in all.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to Shield
PHENOTYPE:
Fish:
Observed In:
Stage: 30%-epiboly

Unillustrated author statements

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Dome
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 224(2), Melby, A.E., Beach, C., Mullins, M., and Kimelman, D., Patterning the early zebrafish by the opposing actions of bozozok and vox/vent, 275-285, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.