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Gene/Allele: Enter one or more terms.
Type at least two characters for each term.
Your search terms will be compared to alleles, transgenic lines,
morpholinos, transgenic constructs and their associated genes.
Previous names will also be searched - e.g. "krox20" will match mutants and knockdowns of the gene egr2b.
Matches are by "begins with" but not "contained in".
As a result, "shh gfp" (without quotes) will return shha and shhb mutants / knockdowns
in transgenic fish that have GFP in the construct,
but not alleles or knockdowns of the hypothetical gene "abcshh" or transgenics with EGFP.
- Note: To find transgenic fish with any fluorescent
reporter gene - GFP, EGFP, Kaede etc., enter "fluorescent" or "fluorescent protein" in the gene
/ allele field.
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Phenotype: Enter terms one at a time in the Phenotype field.
Typing in this field will auto-complete with suggested terms from the Zebrafish Anatomical Ontology (kidney, brain ...)
and from the Gene Ontology (mitosis, mitochondrion ...).
The search will find Fish that have phenotypes in EVERY term you enter (i.e. this is an "AND" search).
Fish with phenotypes in substructures are returned as well - a search for brain will return fish that
have phenotypes in the cerebellum.
- Note: ZFIN does not record phenotypes for reporter gene
expression in an otherwise normal anatomical structure (e.g. GFP in a morphologically normal
heart). To find fish with reporter gene expression in a structure, go to the Gene Expression
Search page, enter the anatomy structure in the Anatomy Terms field and select the option: "Show
only reporter genes in transgenic fish".
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Knockdown Reagent:
Knockdowns refer to zebrafish injected with sequence-targeted
reagents that alter the levels of gene products. Morpholinos (MOs),
TALENs and CRISPRs are examples. Fish that inherit germline
mutations made using TALENS or CRISPRs are not considered knockdowns.
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Sorting your results:
Search results are Fish - the combination of a genotype with knockdown reagents. Three different sorting
schemes can be selected from the "Sort by" pull-down menu at the top-left of the results.
- Fish (Best Match): the default sorting order, optimized for exact
matches in simpler fish.
- Affected gene: single mutant/knockdowns first, then doubles etc.
- Line/Reagent: single allele/transgenic lines first, then doubles etc.
etc.
If you have questions or suggestions, please contact us.