PUBLICATION

Histopathological, genotoxic, and behavioral damages induced by manganese (II) in adult zebrafish

Authors
Zimmermann Prado Rodrigues, G., Staudt, L.B.M., Moreira, M.G., Dos Santos, T.G., de Souza, M.S., Lúcio, C.J., Panizzon, J., Kayser, J.M., Simões, L.A.R., Ziulkoski, A.L., Bonan, C.D., de Oliveira, D.L., Gehlen, G.
ID
ZDB-PUB-200214-2
Date
2020
Source
Chemosphere   244: 125550 (Journal)
Registered Authors
Bonan, Carla Denise
Keywords
Biomarkers, Ecotoxicology, Polluting metals
MeSH Terms
  • Animals
  • Behavior, Animal/drug effects*
  • Chlorides
  • Comet Assay
  • DNA Damage
  • Ecosystem
  • Liver/drug effects
  • Manganese/toxicity*
  • Manganese Compounds
  • Micronucleus Tests
  • Water Pollutants, Chemical/analysis
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/physiology
PubMed
32050344 Full text @ Chemosphere
Abstract
Manganese is a metal often found as an environmental pollutant and very associated with neurological disorders when in high concentrations. However, little is known about the effects that this contaminant can cause when in environmentally relevant concentrations and occurrence, that is, much lower than those commonly studied. So, the aim of the study was to evaluate the effects that environmentally relevant concentrations of this metal would cause in different zebrafish organs (brain, liver, and blood). Acute 96-h and chronic 30-day exposures were performed using the manganese chloride salt as a pollutant. Behavioral alterations of anxiogenic type were observed in the animals after chronic exposures to 4.0 mg L-1 MnCl2, which traveled a greater distance at the bottom of the aquarium. This may be associated with neuronal damages in the telencephalic region responsible for motor and cognitive activity of the fish, observed in animals from the same exposure. In addition, hepatic histopathological damage as vacuolization of hepatocytes and genotoxic damage, identified by comet assay and micronucleus test, was also observed after acute and chronic exposure, especially at the highest pollutant concentrations (8.0 and 16.0 mg L-1 in acute exposure, and 4.0 mg L-1 in chronic exposure. The study reinforces the risk that environmental pollutants pose to the ecosystem, even in low concentrations.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping