PUBLICATION
Agathisflavone isolated from Schinus polygamus (Cav.) Cabrera leaves prevents scopolamine-induced memory impairment and brain oxidative stress in zebrafish (Danio rerio)
- Authors
- Dumitru, G., El-Nashar, H.A.S., Mostafa, N.M., Eldahshan, O.A., Boiangiu, R.S., Todirascu-Ciornea, E., Hritcu, L., Singab, A.N.B.
- ID
- ZDB-PUB-190323-10
- Date
- 2019
- Source
- Phytomedicine : international journal of phytotherapy and phytopharmacology 58: 152889 (Journal)
- Registered Authors
- Keywords
- Agathisflavone, Alzheimer's disease, Memory, Oxidative stress, Schinus polygamus, Scopolamine
- MeSH Terms
-
- Amnesia/chemically induced
- Amnesia/drug therapy
- Anacardiaceae/chemistry*
- Animals
- Antioxidants/pharmacology*
- Biflavonoids/isolation & purification
- Biflavonoids/pharmacology*
- Brain/drug effects*
- Brain/metabolism
- Cognition/drug effects
- Disease Models, Animal
- Female
- Male
- Memory Disorders/chemically induced
- Memory Disorders/prevention & control*
- Oxidative Stress/drug effects
- Plant Leaves/chemistry
- Scopolamine/toxicity*
- Zebrafish
- PubMed
- 30901660 Full text @ Phytomedicine
Citation
Dumitru, G., El-Nashar, H.A.S., Mostafa, N.M., Eldahshan, O.A., Boiangiu, R.S., Todirascu-Ciornea, E., Hritcu, L., Singab, A.N.B. (2019) Agathisflavone isolated from Schinus polygamus (Cav.) Cabrera leaves prevents scopolamine-induced memory impairment and brain oxidative stress in zebrafish (Danio rerio). Phytomedicine : international journal of phytotherapy and phytopharmacology. 58:152889.
Abstract
Background Agathisflavone, a biflavonoid isolated from Schinus polygamus (Cav.) Cabrera leaves been reported to promote various biological activities such as anti-inflammatory properties, promoting cognition and preventing cancer, antioxidant and antiapoptotic activities.
Purpose Here, we tested the hypothesis whether anxiety, amnesia, and brain oxidative stress induced by scopolamine could be counteracted in zebrafish model by agathisflavone and tried to ascertain the underlying mechanism.
Methods Agathisflavone (1, 3 and 5 µg/l) was administered by immersion to zebrafish once daily for 8 days period. Anxiety and memory impairment were induced with scopolamine (100 µM) and measured with the novel tank diving test (NTT) and the Y-maze test. Zebrafish were divided into seven groups (n = 20/group): first group - control, second group - scopolamine (100 μM), the third, fourth and fifth group - agathisflavone treatment groups (FAB, 1 µg/l, 3 µg/l, and 5 µg/l), the sixth group - imipramine (IMP, 20 mg/l, as the positive control in NTT test), and the seventh group - donepezil group (DP, 10 mg/l, as the positive control in Y-maze test). The identification of the agathisflavone was done by spectroscopy, and the structure of the compound was confirmed by (-) Electrospray Ionisation Mass Spectrometry (ESI-MS). The brain oxidative status and acetylcholinesterase (AChE) activity were also investigated.
Results Agathisflavone from Schinus polygamus (Cav.) Cabrera leaves was identified. Also, we demonstrated that agathisflavone significantly reversed scopolamine-induced behavioral score alteration in the NTT and Y-maze tests. Consequently, agathisflavone promoted inhibition of AChE activity and restored the brain antioxidant status.
Conclusion Our results demonstrate that agathisflavone promotes brain antioxidant action and ameliorates scopolamine-induced anxiety and memory deficits in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping