PUBLICATION

Zuogui Pill improves the dexamethasone-induced osteoporosis progression in zebrafish larvae

Authors
Yin, H., Wang, S., Zhang, Y., Wu, M., Wang, J., Ma, Y.
ID
ZDB-PUB-171117-1
Date
2017
Source
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie   97: 995-999 (Journal)
Registered Authors
Keywords
Osteoporosis, Smad-3, TGF-β, Zuogui Pill
MeSH Terms
  • Animals
  • Blotting, Western
  • Bone Density/drug effects*
  • Calcification, Physiologic/drug effects
  • Collagen Type I/genetics
  • Collagen Type II/genetics
  • Dexamethasone/toxicity*
  • Disease Progression
  • Drugs, Chinese Herbal/pharmacology*
  • Larva
  • Osteoporosis/chemically induced
  • Osteoporosis/prevention & control*
  • Phosphorylation/drug effects
  • Signal Transduction/drug effects
  • Smad Proteins/metabolism
  • Transforming Growth Factor beta/metabolism
  • Transforming Growth Factor beta1/metabolism
  • Zebrafish
PubMed
29136778 Full text @ Biomed. Pharmacother.
Abstract
The current study was to evaluate the protective effects of Chinese medicine prescription Zuogui Pill (ZGP) on osteoporosis (OP) in zebrafish larvae induced by dexamethasone. Alizarin Red staining, calcium and phosphorus determination were performed to evaluate the effect of ZGP on bone mineralization. Hydroxyproline (HP), Alkaline phosphatase (ALP), and tartrate resistant acid phosphatase (TRAP) were also measured by commercial kits. We found that ZGP had positive effects in increasing bone mineral content (BMC), strengthening bone biomechanical, promoting bone formation, inhibiting bone resorption, and mediating protein levels of TGF-β1/Smads signaling pathway. The findings demonstrated that ZGP treatments inhibited the phosphorylation of TGF-β and p-Smad 3 as well as the expressions of collagen I and collagen II by western blot. Taken together, we demonstrated that ZGP may prevent osteoporosis via reversing the imbalance of bone fomation/bone resorption and activating the TGF-β-Smad signal.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping