PUBLICATION

Dissection of organs from the adult zebrafish

Authors
Gupta, T., and Mullins, M.C.
ID
ZDB-PUB-100309-19
Date
2010
Source
Journal of visualized experiments : JoVE   (37): (Journal)
Registered Authors
Gupta, Tripti, Mullins, Mary C.
Keywords
none
MeSH Terms
  • Animals
  • Dissection/methods*
  • Female
  • Male
  • Zebrafish/anatomy & histology
  • Zebrafish/surgery*
PubMed
20203557 Full text @ J. Vis. Exp.
Abstract
Over the last 20 years, the zebrafish has become a powerful model organism for understanding vertebrate development and disease. Although experimental analysis of the embryo and larva is extensive and the morphology has been well documented, descriptions of adult zebrafish anatomy and studies of development of the adult structures and organs, together with techniques for working with adults are lacking. The organs of the larva undergo significant changes in their overall structure, morphology, and anatomical location during the larval to adult transition. Externally, the transparent larva develops its characteristic adult striped pigment pattern and paired pelvic fins, while internally, the organs undergo massive growth and remodeling. In addition, the bipotential gonad primordium develops into either testis or ovary. This protocol identifies many of the organs of the adult and demonstrates methods for dissection of the brain, gonads, gastrointestinal system, heart, and kidney of the adult zebrafish. The dissected organs can be used for in situ hybridization, immunohistochemistry, histology, RNA extraction, protein analysis, and other molecular techniques. This protocol will assist in the broadening of studies in the zebrafish to include the remodeling of larval organs, the morphogenesis of organs specific to the adult and other investigations of the adult organ systems.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping