PUBLICATION
Antipsychotic drugs inhibit nucleotide hydrolysis in zebrafish (Danio rerio) brain membranes
- Authors
- Seibt, K.J., Oliveira, R.D., Rico, E.P., Dias, R.D., Bogo, M.R., and Bonan, C.D.
- ID
- ZDB-PUB-081114-6
- Date
- 2009
- Source
- Toxicology in vitro : an international journal published in association with BIBRA 23(1): 78-82 (Journal)
- Registered Authors
- Bonan, Carla Denise
- Keywords
- Haloperidol, Sulpiride, Olanzapine, Antipsychotic, Schizophrenia, Ectonucleotidase, Zebrafish
- MeSH Terms
-
- 5'-Nucleotidase/antagonists & inhibitors
- 5'-Nucleotidase/metabolism*
- Animals
- Antipsychotic Agents/pharmacology
- Benzodiazepines/pharmacology
- Brain/drug effects*
- Brain/enzymology
- Dose-Response Relationship, Drug
- Enzyme Inhibitors/pharmacology*
- Female
- Haloperidol/pharmacology
- Hydrolysis
- Intracellular Membranes/drug effects*
- Intracellular Membranes/enzymology
- Male
- Nucleoside-Triphosphatase/antagonists & inhibitors
- Nucleoside-Triphosphatase/metabolism*
- Sulpiride/pharmacology
- Zebrafish
- PubMed
- 18996465 Full text @ Toxicol. In Vitro
Citation
Seibt, K.J., Oliveira, R.D., Rico, E.P., Dias, R.D., Bogo, M.R., and Bonan, C.D. (2009) Antipsychotic drugs inhibit nucleotide hydrolysis in zebrafish (Danio rerio) brain membranes. Toxicology in vitro : an international journal published in association with BIBRA. 23(1):78-82.
Abstract
Haloperidol (HAL), olanzapina (OLZ), and sulpirida (SULP) are antipsychotic drugs widely used in the pharmacotherapy of psychopathological symptoms observed in schizophrenia or mood-related psychotic symptoms in affective disorders. Here, we tested the in vitro effects of different concentrations of a typical (HAL) and two atypical (OLZ and SULP) antipsychotic drugs on ectonucleotidase activities from zebrafish brain membranes. HAL inhibited ATP (28.9%) and ADP (26.5%) hydrolysis only at 250muM. OLZ decreased ATPase activity at all concentrations tested (23.8-60.7%). SULP did not promote significant changes on ATP hydrolysis but inhibited ADP hydrolysis at 250muM (25.6%). All drugs tested, HAL, OLZ, and SULP, did not promote any significant changes on 5'-nucleotidase activity in the brain membranes of zebrafish. These findings demonstrated that antipsychotic drugs could inhibit NTPDase activities whereas did not change 5'-nucleotidase. Such modulation can alter the adenosine levels, since the ectonucleotidase pathway is an important source of extracellular adenosine. Thus, it is possible to suggest that changes promoted by antipsychotic drugs in the bilayer membrane could alter the NTPDase activities, modulating extracellular ATP and adenosine levels.
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