Figure 4

Effect of banaba leaf extract and policosanol (alone or in combination) supplementation on the tail fin regeneration and blood glucose level of the hyperlipidemic zebrafish injected with streptozotocin (n = 12). (A) Pictorial view of amputated tail fin regeneration (tissue regeneration) after 14 days. The blue arrow indicates the tail fissure, while the red line depicts the regenerated tail fin area after 14 days. (B) A time-dependent (0–14 days) tail fin regenerated area was observed among the different treatment groups. The symbol *** (p < 0.001) indicates the statistical significance of the difference between groups vs the group injected with EtOH+CML, as determined by the two-way ANOVA, followed by Tukey’s post-hoc analysis. (C) The blood glucose level was examined after 14 days. Abbreviations: HCD (high cholesterol diet), STZ (streptozotocin), BNB (banaba), and PCO (policosanol). The symbols **, and *** underline the statistical significance at p < 0.01, and 0.001, respectively, vs. the HCD+STZ group. † indicate statistical significance at p < 0.05 in relation to the BNB+PCO group; ns represents a non-significant (p > 0.05) difference.