PUBLICATION
Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2
- Authors
- Tokarz, J., Mindnich, R., Norton, W., Möller, G., Hrabé de Angelis, M., and Adamski, J.
- ID
- ZDB-PUB-111117-27
- Date
- 2012
- Source
- Molecular and Cellular Endocrinology 349(2): 202-13 (Journal)
- Registered Authors
- Norton, Will
- Keywords
- cortisol, 20beta-HSD, zebrafish, metabolism, stress
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Cortisone/metabolism
- Cortisone Reductase/genetics
- Cortisone Reductase/metabolism*
- Endoplasmic Reticulum/metabolism
- Female
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Gene Expression Regulation, Developmental
- Genetic Association Studies
- HEK293 Cells
- HeLa Cells
- Humans
- Hydrocortisone/metabolism*
- Isoenzymes/genetics
- Isoenzymes/metabolism
- Molecular Sequence Data
- Phylogeny
- Sequence Homology, Amino Acid
- Transfection
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- PubMed
- 22061621 Full text @ Mol. Cell. Endocrinol.
Citation
Tokarz, J., Mindnich, R., Norton, W., Möller, G., Hrabé de Angelis, M., and Adamski, J. (2012) Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2. Molecular and Cellular Endocrinology. 349(2):202-13.
Abstract
Hydroxysteroid dehydrogenases (HSDs) are involved in metabolism and pre-receptor regulation of steroid hormones. While 17beta-HSDs and 11beta-HSDs are extensively studied in mammals, only few orthologs are characterized in fish. We discovered a novel zebrafish HSD candidate closely related to 17beta-HSD types 3 and 12, which has orthologs in other species. The enzyme catalyzes the conversion of cortisone to 20beta-hydroxycortisone identified by LC-MS/MS. We named the new enzyme 20beta-HSD type 2. All 20beta-HSD type 2 orthologs localize in the endoplasmic reticulum. Zebrafish 20beta-HSD type 2 is expressed during embryonic development showing the same expression pattern as 11beta-HSD type 2 known to oxidize cortisol to cortisone. In adult tissues 20beta-HSD type 2 shows a ubiquitous expression pattern with some minor sex-specific differences. In contrast to other enzymes metabolizing C21-steroids and being mostly involved in reproduction we propose that novel type 2 20beta-HSDs in teleost fish are important enzymes in cortisol catabolism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping