PUBLICATION
Zebrafish sp7:EGFP: A transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration
- Authors
- Delaurier, A., Eames, B.F., Blanco-Sánchez, B., Peng, G., He, X., Swartz, M.E., Ullmann, B., Westerfield, M., and Kimmel, C.B.
- ID
- ZDB-PUB-100601-19
- Date
- 2010
- Source
- Genesis (New York, N.Y. : 2000) 48(8): 505-511 (Journal)
- Registered Authors
- Blanco, Bernardo, DeLaurier, April, Eames, Brian F., He, Xinjun, Kimmel, Charles B., Peng, Gang, Swartz, Mary, Ullmann, Bonnie, Westerfield, Monte
- Keywords
- bone, Danio rerio, gene transfer techniques, otic vesicle, regeneration, sp7
- MeSH Terms
-
- Animal Fins/physiology
- Animal Fins/surgery
- Animals
- Animals, Genetically Modified
- Bone Regeneration/genetics*
- Chondrocytes/metabolism
- Chromosomes, Artificial, Bacterial/genetics
- Cloning, Molecular
- Ear/embryology
- Ear/growth & development
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- In Situ Hybridization
- Larva/genetics
- Larva/metabolism
- Organogenesis/genetics*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 20506187 Full text @ Genesis
Citation
Delaurier, A., Eames, B.F., Blanco-Sánchez, B., Peng, G., He, X., Swartz, M.E., Ullmann, B., Westerfield, M., and Kimmel, C.B. (2010) Zebrafish sp7:EGFP: A transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration. Genesis (New York, N.Y. : 2000). 48(8):505-511.
Abstract
We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis. The zinc finger transcription factor sp7 (formerly called osterix) is reported as a marker of osteoblasts. Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212. Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into 1 cell-stage embryos. In this transgenic line, GFP expression reproduces endogenous sp7 gene expression in the otic placode and vesicle, and in forming skeletal structures. GFP-positive cells were also detected in adult fish, and were found associated with regenerating fin rays post-amputation. This line provides an essential tool for the further study of zebrafish otic vesicle formation and the development and regeneration of the skeleton.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping