PUBLICATION
Circadian control by the reduction/oxidation pathway: Catalase represses light-dependent clock gene expression in the zebrafish
- Authors
- Hirayama, J., Cho, S., and Sassone-Corsi, P.
- ID
- ZDB-PUB-071001-10
- Date
- 2007
- Source
- Proceedings of the National Academy of Sciences of the United States of America 104(40): 15747-15752 (Journal)
- Registered Authors
- Sassone-Corsi, Paolo
- Keywords
- light signaling, hydrogen peroxide, transcription, circadian rhythms
- MeSH Terms
-
- Animals
- CLOCK Proteins
- Catalase/genetics*
- Circadian Rhythm*
- Eye Proteins/genetics
- Gene Expression Regulation, Enzymologic*/radiation effects
- Hydrogen Peroxide/metabolism
- Hydrogen Peroxide/pharmacology
- Light
- Oxidation-Reduction
- Period Circadian Proteins
- Reactive Oxygen Species
- Trans-Activators/genetics
- Transcriptional Activation
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 17898172 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Hirayama, J., Cho, S., and Sassone-Corsi, P. (2007) Circadian control by the reduction/oxidation pathway: Catalase represses light-dependent clock gene expression in the zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 104(40):15747-15752.
Abstract
Light is the key entraining stimulus for the circadian clock, but several features of the signaling pathways that convert the photic signal to clock entrainment remain to be deciphered. Here, we show that light induces the production of hydrogen peroxide (H2O2) that acts as the second messenger coupling photoreception to the zebrafish circadian clock. Treatment of light-responsive Z3 cells with H2O2 triggers the induction of zCry1a and zPer2 genes and the subsequent circadian oscillation of zPer1. Remarkably, the induction kinetics and oscillation profile in response to H2O2 are identical to those initiated by light. Catalase (Cat), an antioxidant enzyme degrading H2O2, shows an oscillating pattern of expression and activity, antiphasic to zCry1a and zPer2. Interestingly, overexpression of zCAT results in a reduced light-dependent zCry1a and zPer2 gene induction. In contrast, inhibition of zCAT function enhances light-mediated inducibility of these clock genes. These findings implicate the enzymatic function of zCAT enzyme in the negative regulation of light-dependent clock gene transcriptional activation. Our findings provide an attractive link between the regulation of the cellular reduction/oxidation (redox) state and the photic signaling pathways implicated in circadian control.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping