Research
General Information
ZIRC
ZFIN: EST: IMAGE:7141696
ZFIN ID: ZDB-EST-050411-30

EST Name:IMAGE:7141696


CLONE DATA:
Species: Danio rerio
Library: GISZF001_ra
Cloning Site: Sfi A - Sfi B Digest: Insert Size: 2100
Vector: pDNR-LIB Vector Type: plasmid Polymerase: T3 RNA polymerase
PCR Amplification:
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'
Source:  The I.M.A.G.E. Consortium    (order this)
Thisse in situ hybridization protocol

GENE EXPRESSION:(current status)
Directly submitted expression data: 6 figure(s) (10 images) from Thisse et al., 2004 [IMAGE:7141696]

MARKER RELATIONSHIPS:
IMAGE:7141696 Is encoded by [Gene] eng2b (1)

SEQUENCE INFORMATION:
Type Accession # Length (bp/aa) Analysis
RNA GenBank:CK680198 439 bp

MAPPING INFORMATION:
  None submitted

CITATIONS   (1)