Research
General Information
ZIRC
ZFIN ID: ZDB-LAB-970512-1
University of Notre Dame Center for Zebrafish Research
Department of Biological Sciences
University of Notre Dame
107 Galvin Life Science Center
Notre Dame, IN
46556
USA
Contact Info:
Contact Person: Vihtelic, Thomas
Phone: (574) 631-8641
Fax: (574) 631-7413
Email: vihtelic.3@nd.edu
URL:
Line Designation: nt 		Lab Members:
Hyde, David R. , PI/Director
Thummel, Ryan , Co-PI/Senior Scientist
Vihtelic, Thomas , Co-PI/Senior Scientist
Bailey, Travis , Post-Doc
Kassen, Sean , Post-Doc
Conner, Clay , Graduate Student
Gorsuch, Ryne , Graduate Student
Harding, Rachel , Graduate Student
Montgomery, Jacob , Graduate Student
Murphy, Taylor , Graduate Student
Nelson, Craig , Graduate Student
Raycroft, Fran , Graduate Student
Bang, Debbie , Fish Facility Staff
Heed, Karen , Fish Facility Staff
Raje, Stacey , Fish Facility Staff
Guzicki, Suzyanne , Technical Staff
Statement of Research Interests:

Zebrafish retina and lens molecular genetics.

Zebrafish Publications of Lab members :
Montgomery, J.E., Parsons, M.J., and Hyde, D.R. (2010) A novel model of retinal ablation demonstrates that the extent of rod cell death regulates the origin of the regenerated zebrafish rod photoreceptors. J. Comp. Neurol.. 518(6):800-814
Yang, X., Zou, J., Hyde, D.R., Davidson, L.A., and Wei, X. (2009) Stepwise maturation of apicobasal polarity of the neuroepithelium is essential for vertebrate neurulation. J. Neurosci.. 29(37):11426-11440
Barresi, M.J., Farber, S.A., Hutson, L.D., Hyde, D.R., Liang, J.O., and Morris, J.K. (2008) Zebrafish in the Classroom. Zebrafish. 5(3):205-208
Burket, C.T., Montgomery, J.E., Thummel, R., Kassen, S.C., Lafave, M.C., Langenau, D.M., Zon, L.I., and Hyde, D.R. (2008) Generation and characterization of transgenic zebrafish lines using different ubiquitous promoters. Transgenic. Res.. 17(2):265-279
Harding, R.L., Howley, S., Baker, L.J., Murphy, T.R., Archer, W.E., Wistow, G., Hyde, D.R., and Vihtelic, T.S. (2008) Lengsin expression and function during zebrafish lens formation. Exp. Eye Res.. 86(5):807-818
Hoptak-Solga, A.D., Nielsen, S., Jain, I., Thummel, R., Hyde, D.R., and Iovine, M.K. (2008) Connexin43 (GJA1) is required in the population of dividing cells during fin regeneration. Dev. Biol.. 317(2):541-548
Kassen, S. (2008) Characterization of light-induced zebrafish retinal regeneration and the role of Stat3, CNTF, and Olig2 during light-induced zebrafish retinal regeneration. Ph.D. Thesis, University of Notre Dame. 222p
Kassen, S.C., Thummel, R., Burket, C.T., Campochiaro, L.A., Harding, M.J., and Hyde, D.R. (2008) The Tg(ccnb1:EGFP) transgenic zebrafish line labels proliferating cells during retinal development and regeneration. Mol. Vis.. 14:951-963
Sherpa, T., Fimbel, S.M., Mallory, D.E., Maaswinkel, H., Spritzer, S.D., Sand, J.A., Li, L., Hyde, D.R., and Stenkamp, D.L. (2008) Ganglion cell regeneration following whole-retina destruction in zebrafish. Dev. Neurobiol.. 68(2):166-181
Stenkamp, D.L., Satterfield, R., Muhunthan, K., Sherpa, T., Vihtelic, T.S., and Cameron, D. (2008) Age-Related Cone Abnormalities in Zebrafish with Genetic Lesions in Sonic Hedgehog. Invest. Ophthalmol. Vis. Sci.. 49(10):4631-4640
Thummel, R., Kassen, S.C., Enright, J.M., Nelson, C.M., Montgomery, J.E., and Hyde, D.R. (2008) Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration. Exp. Eye Res.. 87(5):433-444
Thummel, R., Kassen, S.C., Montgomery, J.E., Enright, J.M., and Hyde, D.R. (2008) Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina. Dev. Neurobiol.. 68(3):392-408
Vihtelic, T.S. (2008) Chapter 6 teleost lens development and degeneration. Int. Rev. Cell Mol. Biol.. 269:341-373
Yin, V.P., Thomson, J.M., Thummel, R., Hyde, D.R., Hammond, S.M., and Poss, K.D. (2008) Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish. Genes Dev.. 22(6):728-733
Alvarez, Y., Cederlund, M.L., Cottell, D.C., Bill, B.R., Ekker, S.C., Torres-Vázquez, J., Weinstein, B.M., Hyde, D.R., Vihtelic, T.S., and Kennedy, B.N. (2007) Genetic determinants of hyaloid and retinal vasculature in zebrafish. BMC Dev. Biol.. 7(1):114
Fimbel, S.M., Montgomery, J.E., Burket, C.T., and Hyde, D.R. (2007) Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish. J. Neurosci.. 27(7):1712-1724
Kassen, S.C., Ramanan, V., Montgomery, J.E., Burket, C.T., Liu, C.G., Vihtelic, T.S., and Hyde, D.R. (2007) Time course analysis of gene expression during light-induced photoreceptor cell death and regeneration in albino zebrafish. Dev. Neurobiol.. 67(8):1009-1031
Tawk, M., Araya, C., Lyons, D.A., Reugels, A.M., Girdler, G.C., Bayley, P.R., Hyde, D.R., Tada, M., and Clarke, J.D. (2007) A mirror-symmetric cell division that orchestrates neuroepithelial morphogenesis. Nature. 446(7137):797-800
Thummel, R., Ju, M., Sarras, M.P. Jr., and Godwin, A.R. (2007) Both Hoxc13 orthologs are functionally important for zebrafish tail fin regeneration. Dev. Genes Evol.. 217(6):413-420
Yu, C.J., Gao, Y., Willis, C.L., Li, P., Tiano, J.P., Nakamura, P.A., Hyde, D.R., and Li, L. (2007) Mitogen-associated protein kinase- and protein kinase A-dependent regulation of rhodopsin promoter expression in zebrafish rod photoreceptor cells. J. Neurosci. Res.. 85(3):488-496
Semina, E.V., Bosenko, D.V., Zinkevich, N.C., Soules, K.A., Hyde, D.R., Vihtelic, T.S., Willer, G.B., Gregg, R.G., and Link, B.A. (2006) Mutations in laminin alpha 1 result in complex, lens-independent ocular phenotypes in zebrafish. Dev. Biol.. 299(1):63-77
Shi, X., Luo, Y., Howley, S., Dzialo, A., Foley, S., Hyde, D.R., and Vihtelic, T.S. (2006) Zebrafish foxe3: Roles in ocular lens morphogenesis through interaction with pitx3. Mech. Dev.. 123(10):761-782
Smith, A.A., Wyatt, K., Vacha, J., Vihtelic, T.S., Samuel Zigler, J. Jr, Wistow, G.J., and Posner, M. (2006) Gene duplication and separation of functions in alphaB-crystallin from zebrafish (Danio rerio). FEBS J.. 273(3):481-490
Thummel, R., Bai, S., Sarras, M.P. Jr, Song, P., McDermott, J., Brewer, J., Perry, M., Zhang, X., Hyde, D.R., and Godwin, A.R. (2006) Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxb. Dev. Dyn.. 235(2):336-346
Thummel, R., Burket, C.T., and Hyde, D.R. (2006) Two different transgenes to study gene silencing and re-expression during zebrafish caudal fin and retinal regeneration. ScientificWorldJournal. 6(1):65-81
Vihtelic, T.S., Soverly, J.E., Kassen, S.C., and Hyde, D.R. (2006) Retinal regional differences in photoreceptor cell death and regeneration in light-lesioned albino zebrafish. Exp. Eye Res.. 82(4):558-575
Wei, X., Luo, Y., and Hyde, D.R. (2006) Molecular cloning of three zebrafish lin7 genes and their expression patterns in the retina. Exp. Eye. Res.. 82(1):122-131
Bai, S., Thummel, R., Godwin, A.R., Nagase, H., Itoh, Y., Li, L., Evans, R., McDermott, J., Seiki, M., and Sarras, M.P. Jr. (2005) Matrix metalloproteinase expression and function during fin regeneration in zebrafish: Analysis of MT1-MMP, MMP2 and TIMP2. Matrix Biol.. 24(4):247-260
Shi, X., Bosenko, D.V., Zinkevich, N.S., Foley, S., Hyde, D.R., Semina, E.V., and Vihtelic, T.S. (2005) Zebrafish pitx3 is necessary for normal lens and retinal development. Mech. Dev.. 122(4):513-527
Thummel, R., Burket, C.T., Brewer, J.L., Sarras, M.P. Jr., Li, L., Perry, M., McDermott, J.P., Sauer, B., Hyde, D.R., and Godwin, A.R. (2005) Cre-mediated site-specific recombination in zebrafish embryos. Dev. Dyn.. 233(4):1366-1377
Vihtelic, T.S., Fadool, J.M., Gao, J., Thornton, K.A., Hyde, D.R., and Wistow, G. (2005) Expressed sequence tag analysis of zebrafish eye tissues for NEIBank. Mol. Vis.. 11:1083-1100
Vihtelic, T.S., Yamamoto, Y., Springer, S.S., Jeffery, W.R., and Hyde, D.R. (2005) Lens opacity and photoreceptor degeneration in the zebrafish lens opaque mutant. Dev. Dyn.. 233(1):52-65
Wistow, G., Wyatt, K., David, L., Gao, C., Bateman, O., Bernstein, S., Tomarev, S., Segovia, L., Slingsby, C., and Vihtelic, T. (2005) gammaN-crystallin and the evolution of the betagamma-crystallin superfamily in vertebrates. FEBS J.. 272(9):2276-2291
Thummel, R. (2004) Genetic analysis of Hoxc13 orthologs in mice and zebrafish. Ph.D. Thesis, University of Kansas, Molecular and Integrative Physiology. 200P
Thummel, R., Li, L., Tanase, C., Sarras, M.P. Jr., and Godwin, A.R. (2004) Differences in expression pattern and function between zebrafish hoxc13 orthologs: recruitment of Hoxc13b into an early embryonic role.. Dev. Biol.. 274(2):318-333
Wei, X., Cheng, Y., Luo, Y., Shi, X., Nelson, S., and Hyde, D.R. (2004) The zebrafish Par-3 homolog is required for separation of the eye fields and retinal lamination, but not neuronal specification. Dev. Biol.. 269(1):286-301
Thummel, R., Li, L., Sarras, M.P., and Godwin, A.R. (2002) Hoxc13 orthologs in zebrafish. Dev. Biol.. 247(2):445
Vihtelic, T.S. and Hyde, D.R. (2002) Zebrafish mutagenesis yields eye morphological mutants with retinal and lens defects. Vision Res.. 42(4):535-540
Kennedy, B.N., Vihtelic, T.S., Checkley, L., Vaughan, K.T., and Hyde, D.R. (2001) Isolation of a zebrafish rod opsin promoter to generate a transgenic zebrafish line expressing EGFP in rod photoreceptors. J. Biol. Chem.. 276(17):14037-14043
Vihtelic, T.S., Huijbregts, R.P., Watkins, W.A., Bankaitis, V.A., and Hyde, D.R. (2001) Identification and characterization of the zebrafish PITP beta: a protein expressed at high levels in the double cone cell synapse. Invest. Ophthalmol. Vis. Sci.. 42(4):1036
Vihtelic, T.S., Yamamoto, Y., Sweeney, M.T., Jeffery, W.R., and Hyde, D.R. (2001) Arrested differentiation and epithelial cell degeneration in zebrafish lens mutants. Dev. Dyn.. 222(4):625-636
Elagin, V.A., Elagina, R.B., Doro, C.J., Vihtelic, T.S., and Hyde, D.R. (2000) Cloning and tissue localization of a novel zebrafish RdgB homolog that lacks a phospholipid transfer domain. Vis. Neurosci.. 17(2):303-311
Vihtelic, T.S. and Hyde, D.R. (2000) Light-induced rod and cone cell death and regeneration in the adult albino zebrafish (Danio rerio) retina. J. Neurobiol.. 44(3):289-307
Vihtelic, T.S., Doro, C.J., and Hyde, D.R. (1999) Cloning and characterization of six zebrafish photoreceptor opsin cDNAs and immunolocalization of their corresponding proteins. Vis. Neurosci.. 16:571-585