PUBLICATION
Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1
- Authors
- Dohrmann, C.E., Kessler, D.S., and Melton, D.A.
- ID
- ZDB-PUB-961014-231
- Date
- 1996
- Source
- Developmental Biology 175: 108-117 (Journal)
- Registered Authors
- Melton, Douglas A.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA-Binding Proteins/biosynthesis
- Embryonic Induction*
- Gene Expression Regulation, Developmental
- Glycoproteins/metabolism*
- Goosecoid Protein
- Homeodomain Proteins*
- Mesoderm/physiology*
- Molecular Sequence Data
- Protein Precursors/metabolism
- Protein Processing, Post-Translational
- Repressor Proteins*
- Species Specificity
- Tissue Distribution
- Transcription Factors*
- Transforming Growth Factor beta/metabolism*
- Xenopus
- Xenopus Proteins
- Zebrafish/embryology*
- Zebrafish Proteins
- PubMed
- 8608857 Full text @ Dev. Biol.
Citation
Dohrmann, C.E., Kessler, D.S., and Melton, D.A. (1996) Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1. Developmental Biology. 175:108-117.
Abstract
The zebrafish DVR-1 (zDVR-1) gene, like Xenopus Vg1, is present maternally as an unprocessed precursor protein which is distributed ubiquitously along the future dorsoventral axis. Also, like Vg1, overexpression of zDVR-1 in zebrafish directs synthesis of more precursor, but no processed protein. However, the native zDVR-1 precursor is processed to mature protein when expressed in Xenopus. Like processed Vg1, mature zDVR-1 is a potent inducer of axial mesoderm. The parallels in expression pattern, apparent regulation of protein processing, and mesoderm-inducing activity support the hypothesis that localized protein processing controls production of a dorsal mesoderm inducer in these two species. Furthermore, using mutant mRNAs, we show that cleavage site sequences of the precursor protein are important in regulating protein processing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping