PUBLICATION

Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1

Authors
Dohrmann, C.E., Kessler, D.S., and Melton, D.A.
ID
ZDB-PUB-961014-231
Date
1996
Source
Developmental Biology   175: 108-117 (Journal)
Registered Authors
Melton, Douglas A.
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA-Binding Proteins/biosynthesis
  • Embryonic Induction*
  • Gene Expression Regulation, Developmental
  • Glycoproteins/metabolism*
  • Goosecoid Protein
  • Homeodomain Proteins*
  • Mesoderm/physiology*
  • Molecular Sequence Data
  • Protein Precursors/metabolism
  • Protein Processing, Post-Translational
  • Repressor Proteins*
  • Species Specificity
  • Tissue Distribution
  • Transcription Factors*
  • Transforming Growth Factor beta/metabolism*
  • Xenopus
  • Xenopus Proteins
  • Zebrafish/embryology*
  • Zebrafish Proteins
PubMed
8608857 Full text @ Dev. Biol.
Abstract
The zebrafish DVR-1 (zDVR-1) gene, like Xenopus Vg1, is present maternally as an unprocessed precursor protein which is distributed ubiquitously along the future dorsoventral axis. Also, like Vg1, overexpression of zDVR-1 in zebrafish directs synthesis of more precursor, but no processed protein. However, the native zDVR-1 precursor is processed to mature protein when expressed in Xenopus. Like processed Vg1, mature zDVR-1 is a potent inducer of axial mesoderm. The parallels in expression pattern, apparent regulation of protein processing, and mesoderm-inducing activity support the hypothesis that localized protein processing controls production of a dorsal mesoderm inducer in these two species. Furthermore, using mutant mRNAs, we show that cleavage site sequences of the precursor protein are important in regulating protein processing.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping