PUBLICATION
Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway
- Authors
- Xu, H., Gong, Z., Zhou, S., Yang, S., Wang, D., Chen, X., Wu, J., Liu, L., Zhong, S., Zhao, J., Tang, J.
- ID
- ZDB-PUB-180719-10
- Date
- 2018
- Source
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 48: 569-582 (Journal)
- Registered Authors
- Keywords
- Endometrial carcinoma, Liposomal curcumin, NF-κB
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Caspase 3/metabolism
- Cell Line, Tumor
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Curcumin/chemistry
- Curcumin/pharmacology*
- Curcumin/therapeutic use
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Endometrial Neoplasms/drug therapy
- Endometrial Neoplasms/pathology*
- Female
- Humans
- Larva/drug effects
- Liposomes/chemistry*
- Matrix Metalloproteinase 9/metabolism
- Microscopy, Fluorescence
- NF-kappa B/metabolism*
- Signal Transduction/drug effects*
- Zebrafish/growth & development
- PubMed
- 30021217 Full text @ Cell Physiol. Biochem.
Citation
Xu, H., Gong, Z., Zhou, S., Yang, S., Wang, D., Chen, X., Wu, J., Liu, L., Zhong, S., Zhao, J., Tang, J. (2018) Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 48:569-582.
Abstract
Background/aims Emerging evidence suggests that curcumin possesses chemopreventive properties against various cancers. However, its poor bioavailability limits its clinical application. In this study, we aimed to utilize encapsulation in liposomes (Lipo) as a strategy for the clinical administration of curcumin for endometrial carcinoma (EC).
Methods Curcumin was encapsulated in a liposomal delivery system to prepare a formulation of liposomal curcumin (LC). EC cell lines Ishikawa and HEC-1 were treated with the compound and cell proliferation was measured using MTT assay. Hoechst 33258 staining assay and flow cytometry were used to detect apoptosis of the cells. Wound healing and cell invasion assays were employed to monitor cell motility. Underlying target signaling, such as NF-κB, caspases, and MMPs, were further studied via qRT-PCR and western blot. Thereafter, a zebrafish model was used to assess the toxicity of LC. Finally, a zebrafish transplantation tumor model of EC was grown and treated with LC. Tumors were monitored and harvested to study the expression of NF-κB.
Results The formation of LC was successfully developed with excellent purity and physical properties. In vitro, LC resulted in dose-dependent inhibition of proliferation, induction of apoptosis, and suppression of Ishikawa and HEC-1 cell motility. LC treatment also suppressed the activation and/or expression of NF-κB, caspase-3, and MMP-9. No demonstrable toxicity was found in the zebrafish model and tumors were suppressed after treatment with LC. PCR analysis also showed down-regulated expression of NF-κB.
Conclusions LC was successfully prepared and played biological roles against EC probably through negative regulation of the NF-κB pathway in vitro and in vivo, which demonstrates its potential therapeutic effects in EC.
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Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping