PUBLICATION
Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells
- Authors
- Uribe, R.A., Hong, S.S., Bronner, M.E.
- ID
- ZDB-PUB-171108-8
- Date
- 2017
- Source
- Developmental Biology 433(1): 17-32 (Journal)
- Registered Authors
- Bronner-Fraser, Marianne, Uribe, Rosa
- Keywords
- Retinoic Acid, enteric nervous system, meis3, neural crest, zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology
- Cell Movement
- Digestive System
- Digestive System Physiological Phenomena
- Enteric Nervous System/metabolism
- Neural Crest/metabolism*
- Neural Crest/physiology
- Neuroglia/metabolism
- Neurons/metabolism
- Organogenesis/physiology
- Tretinoin/metabolism*
- Tretinoin/physiology
- Zebrafish/embryology
- Zebrafish/metabolism
- PubMed
- 29108781 Full text @ Dev. Biol.
Citation
Uribe, R.A., Hong, S.S., Bronner, M.E. (2017) Retinoic acid temporally orchestrates colonization of the gut by vagal neural crest cells. Developmental Biology. 433(1):17-32.
Abstract
The enteric nervous system arises from neural crest cells that migrate as chains into and along the primitive gut, subsequently differentiating into enteric neurons and glia. Little is known about the mechanisms governing neural crest migration en route to and along the gut in vivo. Here, we report that Retinoic Acid (RA) temporally controls zebrafish enteric neural crest cell chain migration. In vivo imaging reveals that RA loss severely compromises the integrity and migration of the chain of neural crest cells during the window of time window when they are moving along the foregut. After loss of RA, enteric progenitors accumulate in the foregut and differentiate into enteric neurons, but subsequently undergo apoptosis resulting in a striking neuronal deficit. Moreover, ectopic expression of the transcription factor meis3 and/or the receptor ret, partially rescues enteric neuron colonization after RA attenuation. Collectively, our findings suggest that retinoic acid plays a critical temporal role in promoting enteric neural crest chain migration and neuronal survival upstream of Meis3 and RET in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping