PUBLICATION
miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2
- Authors
- Othman, N., Nagoor, N.H.
- ID
- ZDB-PUB-171028-7
- Date
- 2017
- Source
- International Journal of Oncology 51(6): 1757-1764 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- 3' Untranslated Regions
- A549 Cells
- Adenocarcinoma/enzymology*
- Adenocarcinoma/genetics*
- Animals
- Apoptosis/physiology
- Caspase 3/metabolism
- Gene Silencing
- Heterografts
- Humans
- Lung Neoplasms/enzymology*
- Lung Neoplasms/genetics*
- MicroRNAs/genetics*
- MicroRNAs/metabolism*
- Proto-Oncogene Proteins c-akt/genetics*
- Proto-Oncogene Proteins c-akt/metabolism
- Transfection
- Zebrafish
- PubMed
- 29075783 Full text @ Int. J. Oncol.
Citation
Othman, N., Nagoor, N.H. (2017) miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2. International Journal of Oncology. 51(6):1757-1764.
Abstract
Lung cancer remains a major health problem with a low 5-year survival rate of patients. Recent studies have shown that dysregulation of microRNAs (miRNAs) are prevalent in lung cancer and these aberrations play a significant role in the progression of tumour progression. In the present study, bioinformatics analyses was employed to predict potential miR-608 targets, which are associated with signaling pathways involved in cancer. Luciferase reporter assay identified AKT2 as a novel target of miR-608, and suppression of its protein levels was validated through western blot analysis. Zebrafish embryos were microinjected with cells transfected with miR-608 to elucidate the role of miR-608 in vivo, and immunostained with antibodies to detect activated caspase-3. We present the first evidence that miR-608 behaves as a tumour suppressor in A549 and SK-LU-1 cells through the regulation of AKT2, suggesting that selective targeting of AKT2 via miR-608 may be developed as a potential therapeutic strategy for miRNA-based non-small cell lung cancer (NSCLC) therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping