PUBLICATION
Molecular dynamics of the cryo-EM CFTR structure
- Authors
- Tordai, H., Leveles, I., Hegedűs, T.
- ID
- ZDB-PUB-170805-9
- Date
- 2017
- Source
- Biochemical and Biophysical Research Communications 491(4): 986-993 (Journal)
- Registered Authors
- Keywords
- CFTR, Computational biology, Cystic fibrosis, Molecular dynamics, Transmembrane protein structure
- MeSH Terms
-
- Animals
- Cryoelectron Microscopy*
- Cystic Fibrosis Transmembrane Conductance Regulator/chemistry*
- Cystic Fibrosis Transmembrane Conductance Regulator/metabolism
- Humans
- Molecular Dynamics Simulation*
- Protein Conformation
- Zebrafish
- PubMed
- 28774558 Full text @ Biochem. Biophys. Res. Commun.
Citation
Tordai, H., Leveles, I., Hegedűs, T. (2017) Molecular dynamics of the cryo-EM CFTR structure. Biochemical and Biophysical Research Communications. 491(4):986-993.
Abstract
Cystic fibrosis (CF), a lethal monogenic disease, is caused by mutant variants of the CF transmembrane conductance regulator (CFTR). Recent advances in single molecule cryo-EM methods enabled structural determination of full-length human and zebrafish CFTR, achieving an important milestone for CF drug development. To relate these structures to the gating cycle, we examined its dynamic features using molecular dynamics simulations. Our results show that the nucleotide binding domains (NBDs) in this bottom-open apo conformation exhibit motions related to dimerization and the bottom-closed apo CFTR model indicates opening of NBDs in contrast to transporters. These observations help in understanding the properties of CFTR chloride channel distinct from transporters and in proper interpretation of available structural information on this ABC protein.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping