PUBLICATION
Cyclin F-Mediated Degradation of SLBP Limits H2A.X Accumulation and Apoptosis upon Genotoxic Stress in G2
- Authors
- Dankert, J.F., Rona, G., Clijsters, L., Geter, P., Skaar, J.R., Bermudez-Hernandez, K., Sassani, E., Fenyö, D., Ueberheide, B., Schneider, R., Pagano, M.
- ID
- ZDB-PUB-170831-8
- Date
- 2016
- Source
- Molecular Cell 64: 507-519 (Journal)
- Registered Authors
- Keywords
- DNA damage response, H2A.X, SLBP, apoptosis, canonical histone mRNA metabolism, cyclin F, genotoxic stress, proteasome, ubiquitin
- MeSH Terms
-
- Amino Acid Motifs
- Animals
- Apoptosis
- Binding Sites
- Cell Line, Tumor
- Cyclins/genetics*
- Cyclins/metabolism
- DNA Damage*
- G2 Phase Cell Cycle Checkpoints/genetics*
- Gene Expression Regulation
- HEK293 Cells
- HeLa Cells
- Histones/genetics*
- Histones/metabolism
- Humans
- Mice
- Nuclear Proteins/genetics*
- Nuclear Proteins/metabolism
- Phosphorylation
- Polyribosomes/genetics
- Polyribosomes/metabolism
- Protein Binding
- Proteolysis
- RNA, Messenger/genetics*
- RNA, Messenger/metabolism
- Rats
- Signal Transduction
- Xenopus laevis
- Zebrafish
- mRNA Cleavage and Polyadenylation Factors/genetics*
- mRNA Cleavage and Polyadenylation Factors/metabolism
- PubMed
- 27773672 Full text @ Mol. Cell
Citation
Dankert, J.F., Rona, G., Clijsters, L., Geter, P., Skaar, J.R., Bermudez-Hernandez, K., Sassani, E., Fenyö, D., Ueberheide, B., Schneider, R., Pagano, M. (2016) Cyclin F-Mediated Degradation of SLBP Limits H2A.X Accumulation and Apoptosis upon Genotoxic Stress in G2. Molecular Cell. 64:507-519.
Abstract
SLBP (stem-loop binding protein) is a highly conserved factor necessary for the processing, translation, and degradation of H2AFX and canonical histone mRNAs. We identified the F-box protein cyclin F, a substrate recognition subunit of an SCF (Skp1-Cul1-F-box protein) complex, as the G2 ubiquitin ligase for SLBP. SLBP interacts with cyclin F via an atypical CY motif, and mutation of this motif prevents SLBP degradation in G2. Expression of an SLBP stable mutant results in increased loading of H2AFX mRNA onto polyribosomes, resulting in increased expression of H2A.X (encoded by H2AFX). Upon genotoxic stress in G2, high levels of H2A.X lead to persistent γH2A.X signaling, high levels of H2A.X phosphorylated on Tyr142, high levels of p53, and induction of apoptosis. We propose that cyclin F co-evolved with the appearance of stem-loops in vertebrate H2AFX mRNA to mediate SLBP degradation, thereby limiting H2A.X synthesis and cell death upon genotoxic stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping