PUBLICATION
CAT7 and cat7l long non-coding RNAs Tune Polycomb Repressive Complex 1 Function During Human and Zebrafish Development
- Authors
- Ray, M.K., Wiskow, O., King, M.J., Ismail, N., Ergun, A., Wang, Y., Plys, A.J., Davis, C.P., Kathrein, K., Sadreyev, R., Borowsky, M.L., Eggan, K., Zon, L., Galloway, J.L., Kingston, R.E.
- ID
- ZDB-PUB-160715-8
- Date
- 2016
- Source
- The Journal of biological chemistry 291(37): 19558-72 (Journal)
- Registered Authors
- Galloway, Jenna, Zon, Leonard I.
- Keywords
- CsCl density gradient, PRC1, chromatin, chromatin regulation, development, gene expression, long noncoding RNA (long ncRNA, lncRNA), mammal, polycomb, zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology*
- Gene Expression Regulation, Developmental/physiology*
- HeLa Cells
- Humans
- Mice
- Models, Biological*
- Neurons/metabolism*
- Polycomb Repressive Complex 1/genetics
- Polycomb Repressive Complex 1/metabolism*
- RNA, Long Noncoding/genetics
- RNA, Long Noncoding/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 27405765 Full text @ J. Biol. Chem.
Citation
Ray, M.K., Wiskow, O., King, M.J., Ismail, N., Ergun, A., Wang, Y., Plys, A.J., Davis, C.P., Kathrein, K., Sadreyev, R., Borowsky, M.L., Eggan, K., Zon, L., Galloway, J.L., Kingston, R.E. (2016) CAT7 and cat7l long non-coding RNAs Tune Polycomb Repressive Complex 1 Function During Human and Zebrafish Development. The Journal of biological chemistry. 291(37):19558-72.
Abstract
The essential functions of Polycomb Repressive Complex 1 (PRC1) in development and gene silencing are thought to involve long non-coding RNAs (lncRNAs), but few specific lncRNAs that guide PRC1 activity are known. We screened for lncRNAs which co-precipitate with PRC1 from chromatin and found candidates that impact Polycomb Group protein (PcG)-regulated gene expression in vivo. A novel lncRNA from this screen, CAT7, regulates expression and PcG binding at the MNX1 locus during early neuronal differentiation. CAT7 contains a unique tandem repeat domain which shares high sequence similarity to a non-syntenic zebrafish analog, cat7l. Defects caused by interference of cat7l RNA during zebrafish embryogenesis were rescued by human CAT7 RNA, enhanced by interference of a PRC1 component, and suppressed by interference of a known PRC1 target gene, demonstrating cat7l genetically interacts with a PRC1. We propose a model whereby PRC1 acts in concert with specific lncRNAs, and that CAT7/cat7l represent convergent lncRNAs that independently evolved to tune PRC1 repression at individual loci.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping