PUBLICATION
Microbial modulation of behavior and stress responses in zebrafish larvae
- Authors
- Davis, D.J., Bryda, E.C., Gillespie, C.H., Ericsson, A.C.
- ID
- ZDB-PUB-160525-7
- Date
- 2016
- Source
- Behavioural brain research 311: 219-27 (Journal)
- Registered Authors
- Keywords
- Anxiety, Behavior, Germ-free, Gnotobiotic, Gut-brain axis, Microbiome, Microbiota, Stress, Zebrafish
- MeSH Terms
-
- Animals
- Anxiety/microbiology*
- Anxiety/physiopathology
- Behavior, Animal/physiology
- Enzyme-Linked Immunosorbent Assay
- Hydrocortisone/metabolism
- Larva
- Metagenome
- Microbiota/genetics
- Microbiota/physiology*
- Models, Animal
- Motor Activity/physiology
- Osmotic Pressure/physiology
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Sequence Analysis, DNA
- Stress, Psychological/microbiology*
- Stress, Psychological/physiopathology
- Zebrafish/growth & development
- Zebrafish/microbiology*
- Zebrafish/physiology
- PubMed
- 27217102 Full text @ Behav. Brain Res.
Citation
Davis, D.J., Bryda, E.C., Gillespie, C.H., Ericsson, A.C. (2016) Microbial modulation of behavior and stress responses in zebrafish larvae. Behavioural brain research. 311:219-27.
Abstract
The influence of the microbiota on behavior and stress responses is poorly understood. Zebrafish larvae have unique characteristics that are advantageous for neuroimmune research, however, they are currently underutilized for such studies. Here, we used germ-free zebrafish to determine the effects of the microbiota on behavior and stress testing. The absence of a microbiota dramatically altered locomotor and anxiety-related behavior. Additionally, characteristic responses to an acute stressor were also obliterated in larvae lacking exposure to microbes. Lastly, treatment with the probiotic Lactobacillus plantarum was sufficient to attenuate anxiety-related behavior in conventionally-raised zebrafish larvae. These results underscore the importance of the microbiota in communicating to the CNS via the microbiome-gut-brain axis and set a foundation for using zebrafish larvae for neuroimmune research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping