PUBLICATION
Penetrance of Congenital Heart Disease in a Mouse Model of Down Syndrome Depends on a Trisomic Potentiator of a Disomic Modifier
- Authors
- Li, H., Edie, S., Klinedinst, D., Jeong, J.S., Blackshaw, S., Maslen, C.L., Reeves, R.H.
- ID
- ZDB-PUB-170505-10
- Date
- 2016
- Source
- Genetics 203: 763-70 (Journal)
- Registered Authors
- Keywords
- Down syndrome, congenital heart disease, disomic modifier, trisomic potentiator
- MeSH Terms
-
- Animals
- Cell Adhesion Molecules/genetics
- Cell Adhesion Molecules/metabolism
- Down Syndrome/genetics*
- Down Syndrome/pathology
- Epistasis, Genetic
- Extracellular Matrix Proteins/genetics
- Extracellular Matrix Proteins/metabolism
- Genes, Modifier*
- Heart Defects, Congenital/genetics*
- Heart Defects, Congenital/pathology
- Mice
- Mice, Inbred C57BL
- Penetrance*
- Trisomy
- Zebrafish
- PubMed
- 27029737 Full text @ Genetics
Citation
Li, H., Edie, S., Klinedinst, D., Jeong, J.S., Blackshaw, S., Maslen, C.L., Reeves, R.H. (2016) Penetrance of Congenital Heart Disease in a Mouse Model of Down Syndrome Depends on a Trisomic Potentiator of a Disomic Modifier. Genetics. 203:763-70.
Abstract
Down syndrome (DS) is a significant risk factor for congenital heart disease (CHD), increasing the incidence 50 times over the general population. However, half of people with DS have a normal heart and thus trisomy 21 is not sufficient to cause CHD by itself. Ts65Dn mice are trisomic for orthologs of >100 Hsa21 genes, and their heart defect frequency is significantly higher than their euploid littermates. Introduction of a null allele of Creld1 into Ts65Dn increases the penetrance of heart defects significantly. However, this increase was not seen when the Creld1 null allele was introduced into Ts1Cje, a mouse that is trisomic for about two thirds of the Hsa21 orthologs that are triplicated in Ts65Dn. Among the 23 genes present in three copies in Ts65Dn but not Ts1Cje, we identified Jam2 as necessary for the increased penetrance of Creld1-mediated septal defects in Ts65Dn. Thus, overexpression of the trisomic gene, Jam2, is a necessary potentiator of the disomic genetic modifier, Creld1 No direct physical interaction between Jam2 and Creld1 was identified by several methods. Regions of Hsa21 containing genes that are risk factors of CHD have been identified, but Jam2 (and its environs) has not been linked to heart formation previously. The complexity of this interaction may be more representative of the clinical situation in people than consideration of simple single-gene models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping