PUBLICATION

The inhibitory effects of quercetin on obesity and obesity-induced inflammation by regulation of MAPK signaling

Authors
Seo, M.J., Lee, Y.J., Hwang, J.H., Kim, K.J., Lee, B.Y.
ID
ZDB-PUB-150817-1
Date
2015
Source
The Journal of Nutritional Biochemistry   26(11): 1308-16 (Journal)
Registered Authors
Keywords
Adipogenesis, MAPK signaling, Obesity, Obesity-induced inflammation, Quercetin
MeSH Terms
  • 3T3-L1 Cells/drug effects
  • Adipogenesis/drug effects*
  • Animals
  • Cell Survival/drug effects
  • Cytokines/metabolism
  • Diet, High-Fat/adverse effects
  • Inflammation/drug therapy*
  • Inflammation/metabolism
  • Lipid Metabolism/drug effects
  • Lipogenesis/drug effects
  • MAP Kinase Signaling System/drug effects*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Nitric Oxide/metabolism
  • Obesity/complications
  • Obesity/drug therapy*
  • Obesity/metabolism
  • Quercetin/pharmacology*
  • Transcription Factors/metabolism
  • Zebrafish/metabolism
PubMed
26277481 Full text @ J. Nutr. Biochem.
Abstract
Quercetin is a flavonoid found in fruits, vegetables, leaves and grains. It has inhibitory, antiviral, antiasthma, anticancer and antiinflammatory effects. Research has suggested that obesity is linked to metabolic disorders. In this study, we examined the inhibitory effect of quercetin on lipid accumulation and obesity-induced inflammation using 3T3-L1, RAW264.7, zebrafish and mouse models. Quercetin suppressed protein levels of the key adipogenic factors C/EBPβ, C/EBPα, PPARγ and FABP4 and the TG-synthesis enzymes lipin1, DGAT1 and LPAATθ. Activation of m-TOR and p70S6K, which are related to insulin and adipogenesis, was down-regulated during adipogenesis in 3T3-L1 cells. Recent research suggested that MAPK signaling factors were involved in adipogenesis and inflammation and that the adipokines MCP-1 and TNF-α attracted macrophages into adipose tissue. Our data showed that quercetin inhibited the MAPK signaling factors ERK1/2, JNK and p38MAPK and MCP-1 and TNF-α in adipocytes and macrophages. Quercetin also inhibited secretion of the inflammatory cytokines IL-1β and IL-6 and stimulated that of IL-10, an antiinflammatory cytokine. In this study, we confirmed the inhibitory effects of quercetin in adipogenesis and inflammation using a mouse model. In mice, quercetin reduced body weight (almost 40%) and suppressed expression of adipogenic, lipogenic and inflammation-related cytokines. Our data demonstrated that quercetin inhibits lipid accumulation and obesity-induced inflammation in the cell and animal models. Our study suggested that quercetin may represent a potential therapeutic agent for other metabolic disorders by regulating obesity and obesity-induced inflammation.
Genes / Markers
Figures
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping