PUBLICATION
Genomic and gene expression responses to genotoxic stress in PAC2 zebrafish embryonic cell line
- Authors
- Šrut, M., Bourdineaud, J.P., Štambuk, A., Klobučar, G.I.
- ID
- ZDB-PUB-150123-3
- Date
- 2015
- Source
- Journal of applied toxicology : JAT 35(11): 1381-9 (Journal)
- Registered Authors
- Keywords
- AFLP, Comet assay, DNA repair genes, quantitative RAPD, xenobiotic metabolism
- MeSH Terms
-
- Amplified Fragment Length Polymorphism Analysis
- Animals
- Benzo(a)pyrene/toxicity*
- Cell Line
- Comet Assay
- DNA Damage/drug effects*
- DNA Repair/drug effects
- Oxidative Stress/drug effects
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 25612249 Full text @ J. Appl. Toxicol.
- CTD
- 25612249
Citation
Šrut, M., Bourdineaud, J.P., Štambuk, A., Klobučar, G.I. (2015) Genomic and gene expression responses to genotoxic stress in PAC2 zebrafish embryonic cell line. Journal of applied toxicology : JAT. 35(11):1381-9.
Abstract
PAC2 cell line is, along most of the developed zebrafish cell lines, poorly characterized concerning its response to genotoxicants. To define the PAC2 cell line response to different forms of genotoxic stress, we exposed the cells to model genotoxic agents (benzo[a]pyrene, B[a]P, and ethyl methanesulfonate) and subsequently monitored DNA damage and alterations by using the battery of tests, including the Comet assay, quantitative random-amplified polymorphic DNA and amplified fragment length polymorphism. The expression of several DNA repair (xpc, xpd, hr23b, rad51, msh2) and oxidative stress response (sod (Cu/Zn)) genes was monitored as well. To obtain an indication of the PAC2 cell line metabolizing capacity, the expression of genes belonging to cyp1, cyp2 and cyp3 families was assessed upon exposure to B[a]P. Genotoxic responses were observed in all the used methods, and quantitative random-amplified polymorphic DNA and amplified fragment length polymorphism proved to be more sensitive by revealing DNA alterations even when the Comet assay indicated lack of significant damage. The PAC2 cell line demonstrated basal and B[a]P-induced expression of several cyp genes, suggesting its ability to metabolize indirect acting xenobiotics to a certain point. Based on these results, PAC2 cells seem to be sensitive zebrafish in vitro model in the genotoxicity assessment of the direct acting genotoxicant; however, they are less sensitive toward the indirect acting genotoxicant due to their limited metabolizing properties.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping