PUBLICATION
The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ
- Authors
- Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., Heisenberg, C.
- ID
- ZDB-PUB-141224-8
- Date
- 2014
- Source
- Developmental Cell 31: 774-783 (Journal)
- Registered Authors
- Barone, Vanessa, Behrndt, Martin, Compagnon, Julien, Heisenberg, Carl-Philipp, Rajshekar, Shrivarsha
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning*
- Cell Nucleus/metabolism
- Cell Shape*
- Cilia/physiology
- Epithelial Cells/cytology
- Epithelium/metabolism
- Extracellular Matrix/metabolism
- Gene Expression Regulation, Developmental
- Notochord/embryology*
- Notochord/metabolism
- Somites/metabolism
- Stem Cells/cytology
- Zebrafish/embryology*
- Zebrafish Proteins/metabolism
- PubMed
- 25535919 Full text @ Dev. Cell
Citation
Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., Heisenberg, C. (2014) The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ. Developmental Cell. 31:774-783.
Abstract
Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping