PUBLICATION
A non-inactivating high-voltage-activated two-pore Na⁺ channel that supports ultra-long action potentials and membrane bistability
- Authors
- Cang, C., Aranda, K., Ren, D.
- ID
- ZDB-PUB-170214-289
- Date
- 2014
- Source
- Nature communications 5: 5015 (Journal)
- Registered Authors
- Keywords
- Ion channels in the nervous system, Membrane potential, Synaptic transmission
- MeSH Terms
-
- Action Potentials*
- Amino Acid Sequence
- Animals
- Cell Membrane/metabolism*
- Molecular Sequence Data
- Neurons/metabolism
- Sequence Alignment
- Voltage-Gated Sodium Channels/chemistry
- Voltage-Gated Sodium Channels/genetics
- Voltage-Gated Sodium Channels/metabolism
- Xenopus Proteins/chemistry
- Xenopus Proteins/genetics
- Xenopus Proteins/metabolism
- Xenopus laevis/genetics
- Xenopus laevis/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 25256615 Full text @ Nat. Commun.
Citation
Cang, C., Aranda, K., Ren, D. (2014) A non-inactivating high-voltage-activated two-pore Na⁺ channel that supports ultra-long action potentials and membrane bistability. Nature communications. 5:5015.
Abstract
Action potentials (APs) are fundamental cellular electrical signals. The genesis of short APs lasting milliseconds is well understood. Ultra-long APs (ulAPs) lasting seconds to minutes also occur in eukaryotic organisms, but their biological functions and mechanisms of generation are largely unknown. Here, we identify TPC3, a previously uncharacterized member of the two-pore channel protein family, as a new voltage-gated Na(+) channel (NaV) that generates ulAPs, and that establishes membrane potential bistability. Unlike the rapidly inactivating NaVs that generate short APs in neurons, TPC3 has a high activation threshold, activates slowly and does not inactivate-three properties that help generate long-lasting APs and guard the membrane against unintended perturbation. In amphibian oocytes, TPC3 forms a channel similar to channels induced by depolarization and sperm entry into eggs. TPC3 homologues are present in plants and animals, and they may be important for cellular processes and behaviours associated with prolonged membrane depolarization.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
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