PUBLICATION

A network of epigenetic regulators guides developmental haematopoiesis in vivo

Authors
Huang, H.T., Kathrein, K.L., Barton, A., Gitlin, Z., Huang, Y.H., Ward, T.P., Hofmann, O., Dibiase, A., Song, A., Tyekucheva, S., Hide, W., Zhou, Y., and Zon, L.I.
ID
ZDB-PUB-140101-31
Date
2013
Source
Nature cell biology   15(12): 1516-1525 (Journal)
Registered Authors
Song, Anhua, Zhou, Yi, Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Chromatin/genetics
  • Chromatin/metabolism
  • Chromatin Assembly and Disassembly
  • Epigenesis, Genetic
  • Erythroid Cells/metabolism
  • Gene Knockdown Techniques
  • Gene Regulatory Networks
  • Hematopoiesis/genetics*
  • Hematopoietic Stem Cells/physiology
  • Humans
  • Morpholinos/genetics
  • Protein Interaction Maps
  • Protein Subunits/genetics
  • Protein Subunits/metabolism
  • Reverse Genetics
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
24240475 Full text @ Nat. Cell Biol.
Abstract

The initiation of cellular programs is orchestrated by key transcription factors and chromatin regulators that activate or inhibit target gene expression. To generate a compendium of chromatin factors that establish the epigenetic code during developmental haematopoiesis, a large-scale reverse genetic screen was conducted targeting orthologues of 425 human chromatin factors in zebrafish. A set of chromatin regulators was identified that target different stages of primitive and definitive blood formation, including factors not previously implicated in haematopoiesis. We identified 15 factors that regulate development of primitive erythroid progenitors and 29 factors that regulate development of definitive haematopoietic stem and progenitor cells. These chromatin factors are associated with SWI/SNF and ISWI chromatin remodelling, SET1 methyltransferase, CBP–p300–HBO1–NuA4 acetyltransferase, HDAC–NuRD deacetylase, and Polycomb repressive complexes. Our work provides a comprehensive view of how specific chromatin factors and their associated complexes play a major role in the establishment of haematopoietic cells in vivo.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping