Conserved inhibitory role of teleost SOCS-1s in IFN signaling pathways
- Authors
- Nie, L., Xiong, R., Zhang, Y.S., Zhu, L.Y., Shao, J.Z., and Xiang, L.X.
- ID
- ZDB-PUB-131203-1
- Date
- 2014
- Source
- Developmental and comparative immunology 43(1): 23-29 (Journal)
- Registered Authors
- Keywords
- teleost fish SOCS-1, negative regulator, IFN signal pathway, SOCS-1 variant
- MeSH Terms
-
- Animals
- Conserved Sequence
- Cytokines/genetics
- Cytokines/metabolism
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Genetic Variation
- HeLa Cells
- Humans
- Interferons/metabolism*
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/metabolism*
- Mammals
- Mutation/genetics
- Myxovirus Resistance Proteins/genetics
- Myxovirus Resistance Proteins/metabolism
- Protein Kinases/genetics
- Protein Kinases/metabolism
- Signal Transduction
- Suppressor of Cytokine Signaling Proteins/genetics
- Suppressor of Cytokine Signaling Proteins/metabolism*
- Tetraodontiformes/immunology*
- Transgenes/genetics
- Ubiquitins/genetics
- Ubiquitins/metabolism
- Zebrafish/immunology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 24183820 Full text @ Dev. Comp. Immunol.
The suppressor of cytokine signaling 1 (SOCS-1) protein is a critical regulator in the immune systems of humans and mammals, which functions classically as an inhibitor of the IFN signaling pathways. However, data on functional characterisation of SOCS-1 in ancient vertebrates are limited. In this study, we report the function of teleost SOCS-1s in IFN signaling in fish models (zebrafish and Tetraodon) and human cells. Structurally, teleost SOCS-1s share conserved functional domains with their mammalian counterparts. Functionally, teleost SOCS-1s could be significantly induced upon stimulation with IFN stimulants and zebrafish IFNφ1. Overexpression of teleost SOCS-1s could dramatically suppress IFNφ1-induced Mx, Viperin and PKZ activation in zebrafish, and IFN-induced ISG15 activation in HeLa cells. Furthermore, a SOCS-1 variant that lacks the KIR domain was also characterised. This study demonstrates the conserved negative regulatory role of teleost SOCS-1s in IFN signaling pathways, providing perspective into the functional conservation of SOCS-1 proteins during evolution.