PUBLICATION

Spatial Distribution of Prominin-1 (CD133) - Positive Cells within Germinative Zones of the Vertebrate Brain

Authors
Jászai, J., Graupner, S., Tanaka, E.M., Funk, R.H., Huttner, W.B., Brand, M., and Corbeil, D.
ID
ZDB-PUB-130708-43
Date
2013
Source
PLoS One   8(5): e63457 (Journal)
Registered Authors
Brand, Michael
Keywords
none
MeSH Terms
  • Ambystoma mexicanum
  • Animals
  • Antigens, CD/genetics
  • Antigens, CD/metabolism*
  • Brain/cytology*
  • Brain/embryology
  • Brain/growth & development
  • Chick Embryo
  • Female
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Glycoproteins/genetics
  • Glycoproteins/metabolism*
  • Mice
  • Neural Plate/cytology
  • Neural Stem Cells/metabolism*
  • Neural Tube/cytology
  • Organ Specificity
  • Peptides/genetics
  • Peptides/metabolism*
  • Regeneration
  • Spinal Cord/physiology
  • Up-Regulation
  • Zebrafish
PubMed
23723983 Full text @ PLoS One
Abstract

Background

In mammals, embryonic neural progenitors as well as adult neural stem cells can be prospectively isolated based on the cell surface expression of prominin-1 (CD133), a plasma membrane glycoprotein. In contrast, characterization of neural progenitors in non-mammalian vertebrates endowed with significant constitutive neurogenesis and inherent self-repair ability is hampered by the lack of suitable cell surface markers. Here, we have investigated whether prominin-1–orthologues of the major non-mammalian vertebrate model organisms show any degree of conservation as for their association with neurogenic geminative zones within the central nervous system (CNS) as they do in mammals or associated with activated neural progenitors during provoked neurogenesis in the regenerating CNS.

Methods

We have recently identified prominin-1 orthologues from zebrafish, axolotl and chicken. The spatial distribution of prominin-1–positive cells – in comparison to those of mice – was mapped in the intact brain in these organisms by non-radioactive in situ hybridization combined with detection of proliferating neural progenitors, marked either by proliferating cell nuclear antigen or 5-bromo-deoxyuridine. Furthermore, distribution of prominin-1 transcripts was investigated in the regenerating spinal cord of injured axolotl.

Results

Remarkably, a conserved association of prominin-1 with germinative zones of the CNS was uncovered as manifested in a significant co-localization with cell proliferation markers during normal constitutive neurogenesis in all species investigated. Moreover, an enhanced expression of prominin-1 became evident associated with provoked, compensatory neurogenesis during the epimorphic regeneration of the axolotl spinal cord. Interestingly, significant prominin-1–expressing cell populations were also detected at distinct extraventricular (parenchymal) locations in the CNS of all vertebrate species being suggestive of further, non-neurogenic neural function(s).

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping