Lactobacillus rhamnosus Accelerates Zebrafish Backbone Calcification and Gonadal Differentiation through Effects on the GnRH and IGF Systems
- Authors
- Avella, M.A., Place, A., Du, S.J., Williams, E., Silvi, S., Zohar, Y., and Carnevali, O.
- ID
- ZDB-PUB-121012-16
- Date
- 2012
- Source
- PLoS One 7(9): e45572 (Journal)
- Registered Authors
- Carnevali, Oliana, Du, Shao Jun (Jim), Zohar, Yonathan
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Body Size
- Body Weight
- Bone and Bones/metabolism*
- Calcification, Physiologic*
- Female
- Gastrointestinal Tract/microbiology
- Gonadotropin-Releasing Hormone/metabolism*
- Gonads/metabolism*
- Lacticaseibacillus rhamnosus/physiology*
- Male
- Muscles/metabolism
- Neurons/metabolism
- Pyrrolidonecarboxylic Acid/analogs & derivatives*
- Pyrrolidonecarboxylic Acid/metabolism
- Sex Differentiation/physiology
- Somatomedins/metabolism*
- Stress, Physiological
- Zebrafish/growth & development*
- Zebrafish/microbiology*
- PubMed
- 23029107 Full text @ PLoS One
Endogenous microbiota play essential roles in the host’s immune system, physiology, reproduction and nutrient metabolism. We hypothesized that a continuous administration of an exogenous probiotic might also influence the host’s development. Thus, we treated zebrafish from birth to sexual maturation (2-months treatment) with Lactobacillus rhamnosus, a probiotic species intended for human use. We monitored for the presence of L. rhamnosus during the entire treatment. Zebrafish at 6 days post fertilization (dpf) exhibited elevated gene expression levels for Insulin-like growth factors -I and -II, Peroxisome proliferator activated receptors -α and -β, VDR-α and RAR-γ when compared to untreated-10 days old zebrafish. Using a gonadotropin-releasing hormone 3 GFP transgenic zebrafish (GnRH3-GFP), higher GnRH3 expression was found at 6, 8 and 10 dpf upon L. rhamnosus treatment. The same larvae exhibited earlier backbone calcification and gonad maturation. Noteworthy in the gonad development was the presence of first testes differentiation at 3 weeks post fertilization in the treated zebrafish population -which normally occurs at 8 weeks- and a dramatic sex ratio modulation (93% females, 7% males in control vs. 55% females, 45% males in the treated group). We infer that administration of L. rhamnosus stimulated the IGF system, leading to a faster backbone calcification. Moreover we hypothesize a role for administration of L. rhamnosus on GnRH3 modulation during early larval development, which in turn affects gonadal development and sex differentiation. These findings suggest a significant role of the microbiota composition on the host organism development profile and open new perspectives in the study of probiotics usage and application.