PUBLICATION
Zebrafish Eleutheroembryos Provide a Suitable Vertebrate Model for Screening Chemicals that Impair Thyroid Hormone Synthesis
- Authors
- Thienpont, B., Tingaud-Sequeira, A., Prats, E., Barata, C., Babin, P.J., and Raldúa, D.
- ID
- ZDB-PUB-110803-37
- Date
- 2011
- Source
- Environmental science & technology 45(7): 7525-7532 (Journal)
- Registered Authors
- Babin, Patrick J., Raldúa, Demetrio
- Keywords
- none
- MeSH Terms
-
- Animals
- Antithyroid Agents/pharmacology*
- Biological Assay/methods
- Child
- Embryo, Nonmammalian*/anatomy & histology
- Embryo, Nonmammalian*/drug effects
- Embryo, Nonmammalian*/metabolism
- Female
- Humans
- In Situ Hybridization
- Iodides/metabolism
- Methimazole/pharmacology
- Models, Animal
- Perchlorates/pharmacology
- Potassium Compounds/pharmacology
- Pregnancy
- Thyroid Function Tests
- Thyroid Gland/drug effects*
- Thyroid Gland/metabolism*
- Thyroid Hormones/biosynthesis*
- Water Pollutants, Chemical/pharmacology*
- Zebrafish*/embryology
- Zebrafish*/metabolism
- PubMed
- 21800831 Full text @ Env. Sci. Tech.
- CTD
- 21800831
Citation
Thienpont, B., Tingaud-Sequeira, A., Prats, E., Barata, C., Babin, P.J., and Raldúa, D. (2011) Zebrafish Eleutheroembryos Provide a Suitable Vertebrate Model for Screening Chemicals that Impair Thyroid Hormone Synthesis. Environmental science & technology. 45(7):7525-7532.
Abstract
Thyroxine-Immunofluorescence Quantitative Disruption Test (TIQDT) was designed to provide a simple, rapid, alternative bioassay for assessing the potential of chemical pollutants and drugs to disrupt thyroid gland function. This study demonstrated that zebrafish eleutheroembryos provided a suitable vertebrate model, not only for screening the potential thyroid disrupting effect of molecules, but also for estimating the potential hazards associated with exposure to chemicals directly impairing thyroxine (T4) synthesis. Amitrole, potassium perchlorate, potassium thiocyanate, methimazole (MMI), phloroglucinol, 6-propyl-2-thiouracil, ethylenethiourea, benzophenone-2, resorcinol, pyrazole, sulfamethoxazole, sodium bromide, mancozeb and genistein were classified as thyroid gland function disruptors. Concordance between TIQDT on zebrafish and mammalian published data was very high and the physiological relevance of T4-intrafollicular content was clearly higher than regulation at the transcriptional level of tg or slc5a5. Moreover, concentration-response analysis provided information about the thyroid disrupting potency and hazard of selected positive compounds. Finally, the effect of perchlorate, but not MMI, was completely rescued by low-micromolar amounts of iodide. TIQDT performed on zebrafish eleutheroembryos is an alternative whole-organism screening assay that provides relevant information for environmental and human risk assessments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping