Polyphyllin D, a steroidal saponin from Paris polyphylla, inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo
- Authors
- Chan, J.Y., Koon, J.C., Liu, X., Detmar, M., Yu, B., Kong, S.K., and Fung, K.P.
- ID
- ZDB-PUB-110613-37
- Date
- 2011
- Source
- Journal of ethnopharmacology 137(1): 64-9 (Journal)
- Registered Authors
- Keywords
- Anti-angiogenesis; Human microvascular endothelial cells; Zebrafish; Vascular endothelial growth factor
- MeSH Terms
-
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Cell Cycle/drug effects
- Cell Differentiation/drug effects
- Cell Line
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- DNA Replication/drug effects
- Diosgenin/analogs & derivatives*
- Diosgenin/isolation & purification
- Diosgenin/pharmacology
- Dose-Response Relationship, Drug
- Drugs, Chinese Herbal/isolation & purification
- Drugs, Chinese Herbal/pharmacology*
- Embryo, Nonmammalian/blood supply
- Embryo, Nonmammalian/drug effects*
- Endothelial Cells/drug effects*
- Humans
- Liliaceae*/chemistry
- Neovascularization, Physiologic/drug effects*
- Plants, Medicinal
- Time Factors
- Zebrafish/embryology
- PubMed
- 21658438 Full text @ J. Ethnopharmacol.
AIM OF THE STUDY:
Angiogenesis, the process of blood vessel formation, is critical to tumour growth. The importance of angiogenesis in tumour development has lead to the development of anti-angiogenic strategies to inhibit tumour growth. In this study, polyphyllin D (PD), an active component in Chinese herb, Paris polyphylla, was evaluated for its potential anti-angiogenic effects.
MATERIALS AND METHODS:
The inhibitory effects of PD on three important processes involved in angiogenesis, i.e. proliferation, migration and differentiation were examined using human microvascular endothelial cell line HMEC-1 by MTT assay, scratch assay and tube formation assay, respectively. Using zebrafish embryos as an animal model of angiogenesis, the anti-angiogenic effect of PD was further verified in vivo.
RESULTS:
PD suppressed the growth of HMEC-1 cells at 0.1-0.4µM without toxic effects. At 0.3µM and 0.4µM, PD significantly inhibited endothelial cell migration and capillary tube formation. About 70% of the zebrafish embryos showed defects in intersegmental vessel formation upon treatment with PD at concentrations of 0.156µM and 0.313µM.
CONCLUSION:
The anti-angiogenic effects of PD have been explored in the study which implied a potential therapeutic development of PD in cancer treatment.