PUBLICATION
Ooplasmic segregation in the zebrafish zygote and early embryo: Pattern of ooplasmic movements and transport pathways
- Authors
- Fuentes, R., and Fernández, J.
- ID
- ZDB-PUB-100625-19
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(8): 2172-2189 (Journal)
- Registered Authors
- Keywords
- ooplasmic segregation, early zebrafish development, cytoplasmic movements, zygote development, ooplasmic domains, zygote cytoskeleton, maternal mRNA localization and transportation
- MeSH Terms
-
- Actin Cytoskeleton/metabolism
- Animals
- Biological Transport
- Body Patterning*
- Cell Movement*
- Cytoplasm/metabolism
- Embryo, Nonmammalian
- Female
- Inclusion Bodies/metabolism
- Microtubules/metabolism
- Oocytes/cytology*
- Organelles/metabolism
- RNA, Messenger/metabolism
- Zebrafish
- Zygote/cytology*
- PubMed
- 20568243 Full text @ Dev. Dyn.
Citation
Fuentes, R., and Fernández, J. (2010) Ooplasmic segregation in the zebrafish zygote and early embryo: Pattern of ooplasmic movements and transport pathways. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(8):2172-2189.
Abstract
Patterns of cytoplasmic movements and organization of transport pathways were examined in live or fixed zygotes and early zebrafish embryos using a variety of techniques. The zygote blastodisc grows by accumulation of ooplasm, transported to the animal pole from distinct sectors of ecto- and endoplasm at different speeds and developmental periods, using specific pathways or streamers. Slow transport (5 mum/min) occurs during the first interphase along short streamers, whereas fast transport (9.6-40 mum/min) takes place during the first cleavage division along axial and meridional streamers. Interconnections between streamers allow cargoes to change their speed and final destination. A similar sequence of events occurs during the following divisions. A complex network of microtubules and actin filaments in the endo- and ectoplasm appears to be involved in the transport of inclusions and mRNAs. Actin-dependent intermittent pulsations provoked high-speed back-and-forth movements of cytoplasm that may contribute to redistribution of organelles and maternal determinants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping