PUBLICATION
Tissue-specific transcriptional regulation of monocarboxylate transporters (MCTs) during short-term hypoxia in zebrafish (Danio rerio)
- Authors
- Ngan, A.K., and Wang, Y.S.
- ID
- ZDB-PUB-090828-29
- Date
- 2009
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 154(4): 396-405 (Journal)
- Registered Authors
- Keywords
- MCT, zebrafish, hypoxia, real-time RT-PCR, muscle, brain, gill, heart
- MeSH Terms
-
- Animals
- Carbohydrate Metabolism/genetics
- Citrate (si)-Synthase/genetics
- Gene Expression Regulation*
- Glycolysis
- Hypoxia/genetics*
- L-Lactate Dehydrogenase/genetics
- Monocarboxylic Acid Transporters/genetics*
- Monocarboxylic Acid Transporters/metabolism
- Organ Specificity
- Oxidation-Reduction
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Time Factors
- Transcription, Genetic*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 19709642 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Ngan, A.K., and Wang, Y.S. (2009) Tissue-specific transcriptional regulation of monocarboxylate transporters (MCTs) during short-term hypoxia in zebrafish (Danio rerio). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 154(4):396-405.
Abstract
Monocarboxylate transporters (MCTs) have been shown to be important in regulating metabolism during hypoxia in mammals. However, the role of MCTs in hypoxic survival in lower vertebrates is currently unclear. The goal of this study was to investigate the coordinated responses of MCTs along with other metabolic genes during hypoxia. Therefore, we subjected zebrafish (Danio rerio) to 1.5 mg L(-1) O(2) over 48- and 96-h and measured tissue-specific transcriptional changes of MCTs (1, 2 and 4), lactate dehydrogenase A (LDHa), citrate synthase (CS), and other metabolic genes using real-time RT-PCR. There were no changes in mRNA detected in muscle at 48- and 96-h. When data from both time points were pooled in brain, a significant increase was found in MCT4 (+102%) and LDHa (+28%) mRNA suggesting a preference towards glycolysis. In gills, there were increases in LDHa at 48-h (+101%) and MCT1 (+24%) mRNA from pooled data suggesting that both anaerobic and aerobic metabolism is being utilized. Heart showed the greatest changes in transcriptional regulation compared to other tissues. At 48-h, increases were found in MCT1 (+117%), MCT4 (+86%), LDHa (+197%) mRNA, and pooled data showed an increase in CS (+18%) mRNA. These results suggest that the influx and efflux of lactate are both employed as strategies in cardiac tissue during hypoxia. This study has shown that fish utilize the tissue-specific transcriptional regulation of MCTs along with other metabolic genes during hypoxia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping