PUBLICATION
Kit and foxd3 genetically interact to regulate melanophore survival in zebrafish
- Authors
- Cooper, C.D., Linbo, T.H., and Raible, D.W.
- ID
- ZDB-PUB-090324-10
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(4): 875-886 (Journal)
- Registered Authors
- Linbo, Tor, Raible, David
- Keywords
- melanophore, survival, morphology, kit signaling, foxd3, neural crest, zebrafish
- MeSH Terms
-
- Alleles
- Animals
- Animals, Genetically Modified
- Cell Movement
- Cell Survival
- Female
- Forkhead Transcription Factors/genetics
- Forkhead Transcription Factors/metabolism*
- Gene Expression Regulation, Developmental
- Male
- Melanins/biosynthesis
- Melanophores/cytology*
- Melanophores/metabolism*
- Mutation/genetics
- Proto-Oncogene Proteins c-kit/genetics
- Proto-Oncogene Proteins c-kit/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19301400 Full text @ Dev. Dyn.
Citation
Cooper, C.D., Linbo, T.H., and Raible, D.W. (2009) Kit and foxd3 genetically interact to regulate melanophore survival in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(4):875-886.
Abstract
We have investigated the role of foxd3 activity in conjunction with signaling by the kit tyrosine kinase receptor in zebrafish black pigment cell (melanophore) development. As loss-of-function of these molecules individually has distinct effects on melanophore number, we have examined the phenotype of double mutants. Individuals with a null mutation in kit have fewer melanophores than wild-type, with cells lost through death. When kit mutants are injected with foxd3 antisense morpholino oligonucleotides or crossed with a foxd3 zebrafish mutant, they have more melanophores than their uninjected or foxd3+ counterparts. Examination of foxd3 loss-of-function in two additional kit mutants that differentially alter kit-dependent migration and survival indicates a change in melanophore number in survival mutants only. Consistently, TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling) analysis confirms a partial rescue of melanophores from cell death. Ectopic expression of foxd3 indicates that foxd3 promotes early melanophore death only when kit is inactive. Taken together, these data suggest a kit-dependent role for foxd3 in the regulation of melanophore survival.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping