PUBLICATION
Cold acclimation-induced up-regulation of the ribosomal protein L7 gene in the freeze tolerant wood frog, Rana sylvatica
- Authors
- Wu, S., De Croos, J.N., and Storey, K.B.
- ID
- ZDB-PUB-080826-24
- Date
- 2008
- Source
- Gene 424(1-2): 48-55 (Journal)
- Registered Authors
- Keywords
- cryoprotection, anoxia, dehydration, stress-responsive gene expression, differential display PCR
- MeSH Terms
-
- Acclimatization/genetics
- Amino Acid Sequence
- Animals
- Binding Sites
- Blotting, Northern
- Cloning, Molecular
- Cold Temperature
- DNA, Complementary/genetics
- Freezing
- Humans
- Liver/physiology
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Messenger/genetics
- Ranidae/genetics*
- Ribosomal Proteins/chemistry
- Ribosomal Proteins/genetics*
- Seasons
- Sequence Alignment
- Skin Physiological Phenomena
- PubMed
- 18706984 Full text @ Gene
Citation
Wu, S., De Croos, J.N., and Storey, K.B. (2008) Cold acclimation-induced up-regulation of the ribosomal protein L7 gene in the freeze tolerant wood frog, Rana sylvatica. Gene. 424(1-2):48-55.
Abstract
Natural freezing survival by the wood frog, Rana sylvatica, involves multiple organ-specific changes in gene expression. The present study used differential display PCR to find cold-responsive genes in wood frog skin. A cDNA was retrieved from skin that was in higher amounts in cold- versus warm-acclimated frogs. The cDNA was used to probe a wood frog liver cDNA library and retrieve a long sequence that, after the further application of 5'RACE, was shown to encode the full sequence of the ribosomal large subunit protein 7 (RPL7) (GenBank accession number AF175983). Wood frog RPL7 contained 246 amino acids and shared 90% identity with Xenopus laevis RPL7, 82-83% with chicken and zebrafish homologues, and 79% with mammalian RPL7. Multiple binding domains found in human RPL7 showed differing degrees of conservation in the frog protein. Transcript levels of rpl7 were elevated up to 4-fold in skin of cold-acclimated frogs as compared with warm-acclimated animals. Organ-specific responses by rpl7 transcripts also occurred when frogs were given survivable freezing exposures. Transcripts rose by 1.8-3.3 fold in brain and skeletal muscle during freezing but were unaffected in central organs such as liver and heart. Up-regulation of rpl7 also occurred in brain of anoxia-exposed frogs and RPL7 protein levels increased strongly in heart under both freezing and dehydration stresses. Cold- and freezing-responsive up-regulation of the rpl7 gene and RPL7 protein in selected organs suggests that targeted changes in selected ribosomal proteins may be an integral part of natural freeze tolerance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping