PUBLICATION
Synaptotagmin I and II are present in distinct subsets of central synapses
- Authors
- Fox, M.A., and Sanes, J.R.
- ID
- ZDB-PUB-090520-1
- Date
- 2007
- Source
- The Journal of comparative neurology 503(2): 280-296 (Journal)
- Registered Authors
- Keywords
- synaptic vesicle, retina, bipolar cell, horizontal cell, photoreceptor, calyx of Held
- MeSH Terms
-
- Animals
- Antibodies, Monoclonal/metabolism
- Central Nervous System/cytology
- Central Nervous System/metabolism*
- Cerebellum/cytology
- Cerebellum/metabolism
- Chick Embryo
- Hippocampus/cytology
- Hippocampus/metabolism
- Immunohistochemistry
- Mice
- Photoreceptor Cells/metabolism
- Presynaptic Terminals/metabolism*
- Protein Isoforms
- Retina/cytology
- Retina/metabolism*
- Species Specificity
- Synaptotagmin I/metabolism*
- Synaptotagmin II/metabolism*
- Tissue Distribution
- Vertebrates
- Zebrafish
- PubMed
- 17492637 Full text @ J. Comp. Neurol.
Citation
Fox, M.A., and Sanes, J.R. (2007) Synaptotagmin I and II are present in distinct subsets of central synapses. The Journal of comparative neurology. 503(2):280-296.
Abstract
Synaptotagmin 1 and 2 (syt 1, syt 2) are synaptic vesicle-associated membrane proteins that act as calcium sensors for fast neurotransmitter release from presynaptic nerve terminals. Here we show that widely used monoclonal antibodies, mab 48 and znp-1, stain nerve terminals in multiple species and, in mouse, recognize syt 1 and syt 2, respectively. With these antibodies, we examined the synaptic localization of these synaptotagmin isoforms in the mouse central nervous system. Syt 1 and syt 2 are localized predominantly to different subsets of synapses in retina, hippocampus, cerebellum, and median nucleus of the trapezoid body (MNTB). In the MNTB, syt 1 and syt 2 are present in different presynaptic terminals on the same postsynaptic principal neuron. In retina, horizontal and OFF-bipolar cell terminals contain syt 2, whereas most other terminals contain syt 1. Syt 1 localization in the immature retina resembles that seen in adult; however, syt 2 localization appears strikingly different at perinatal ages and continues to change dramatically prior to eye opening. For example, starburst amacrine cells, which lack syt 2 in adult retina, transiently express syt 2 during the first 2 postnatal weeks. In addition to differences in spatial and temporal distribution, species-specific differences in synaptotagmin localization were observed in retina and cerebellum. The cell-, temporal-, and species-specific expression of synaptotagmin isoforms suggests that each may have distinct functions in neurotransmitter release.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping