PUBLICATION
Molecular cloning of proteasome activator PA28-β subunit of large yellow croaker (Pseudosciana crocea) and its coordinated up-regulation with MHC class I α-chain and β2-microglobulin in poly I:C-treated fish
- Authors
- Liu, G., Zheng, W., and Chen, X.
- ID
- ZDB-PUB-060816-23
- Date
- 2007
- Source
- Molecular immunology 44(6): 1190-1197 (Journal)
- Registered Authors
- Keywords
- Large yellow croaker (Pseudosciana crocea), PA28-β, MHC class I antigen processing and presentation, MHC class I α-chain, β2-microglobulin, Poly I:C
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Fish Proteins/genetics*
- Fish Proteins/isolation & purification
- Histocompatibility Antigens Class I/biosynthesis*
- Histocompatibility Antigens Class I/genetics
- Molecular Sequence Data
- Perciformes/genetics*
- Perciformes/immunology
- Poly I-C/pharmacology*
- Proteasome Endopeptidase Complex/genetics*
- Proteasome Endopeptidase Complex/isolation & purification
- Proteasome Endopeptidase Complex/metabolism
- Protein Subunits/genetics*
- Protein Subunits/isolation & purification
- Up-Regulation*/genetics
- Up-Regulation*/immunology
- beta 2-Microglobulin/biosynthesis*
- beta 2-Microglobulin/genetics
- PubMed
- 16901544 Full text @ Mol. Immunol.
Citation
Liu, G., Zheng, W., and Chen, X. (2007) Molecular cloning of proteasome activator PA28-β subunit of large yellow croaker (Pseudosciana crocea) and its coordinated up-regulation with MHC class I α-chain and β2-microglobulin in poly I:C-treated fish. Molecular immunology. 44(6):1190-1197.
Abstract
Antigenic peptides presented on MHC class I molecules to cytotoxic T-cells are generated in the cytosol by the 20S proteasome. Two activators PA28-alpha and PA28-beta, which are inducible by interferon-gamma (IFN-gamma), activate the latent 20S proteasome, thus playing an important role in the processing of MHC class I antigen. Molecular properties and function in the MHC class I antigen processing of PA28 have been well studied and documented in mammals while little is known in fish. In the present study, we reported the cloning of a PA28-beta gene homologue from the spleen of large yellow croaker (Pseudosciana crocea), an economically important marine fish (LycPA28-beta). The full-length cDNA of LycPA28-beta is 1133 nucleotides (nt) encoding a protein of 245 amino acids (aa), with a putative molecular weight of 27.7kDa. The deduced protein shares 76, 69, 61, 60, 59, 57 and 57% sequence identity to sequences found in zebrafish, flounder, pig, rat, mouse, cattle and human, respectively. The deduced LycPA28-beta contains a PA28-beta subunit-specific insert in the region corresponding to the KEKE motif of the known PA28-alpha (Region B), a conserved activation loop (Region C) and a highly homologous C-terminal region among all three PA28 subunits (Region E), and a characteristic proline-rich motif (Region A) and a potential protein kinase C recognition site (Region D). Western blot analysis of various tissues indicated that LycPA28-beta was constitutively expressed in kidney, liver, spleen and intestine, and weakly expressed in muscle tissue, but not detected in gills, heart and brain. The LycPA28-beta expression was significantly up-regulated in kidney, liver, spleen, intestine and muscle tissues, and also induced in gills after 72h of treatment with a viral micmic, polyinosinic polycytidynic acid (poly I:C). The transcriptional analysis of LycPA28-beta and MHC class I alpha-chain (alpha-chain) and beta(2)-microglobulin (beta(2)m) in spleens of poly I:C-induced large yellow croaker was further performed by RT-PCR. The results showed that the expression of LycPA28-beta and class I alpha-chain and beta(2)m genes was coordinately up-regulated by poly I:C, suggesting that induction of the MHC class I antigen processing and presentation pathway may be required for the antiviral immune response triggered poly I:C in large yellow croaker.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping