PUBLICATION
Identification, characterization, and expression pattern of the chicken EKLF gene
- Authors
- Chervenak, A.P., Basu, P., Shin, M., Redmond, L.C., Sheng, G., and Lloyd, J.A.
- ID
- ZDB-PUB-060517-10
- Date
- 2006
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 235(7): 1933-1940 (Journal)
- Registered Authors
- Shin, Masahiro
- Keywords
- chicken, EKLF, erythroid, transcription factor
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blood Cells/metabolism
- Brain/embryology
- Brain/metabolism
- Chick Embryo
- Conserved Sequence
- Hematopoiesis
- Kruppel-Like Transcription Factors/biosynthesis*
- Kruppel-Like Transcription Factors/genetics
- Molecular Sequence Data
- PubMed
- 16680725 Full text @ Dev. Dyn.
Citation
Chervenak, A.P., Basu, P., Shin, M., Redmond, L.C., Sheng, G., and Lloyd, J.A. (2006) Identification, characterization, and expression pattern of the chicken EKLF gene. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(7):1933-1940.
Abstract
EKLF/KLF1 was the first of the Kruppel-like factors (KLFs) to be identified in mammals and plays an important role in primitive and definitive erythropoiesis. Here, we identify and characterize EKLF in the chicken (cEKLF). The predicted amino acid sequence of the zinc finger region of cEKLF is at least 87.7% similar to mammalian EKLF proteins and is 98.8% and 95% similar to the EKLF orthologues in Xenopus and zebrafish, respectively. During early embryonic development, cEKLF expression is seen in the posterior primitive streak, which gives rise to hematopoietic cells, and then in the blood islands and in circulating blood cells. cEKLF mRNA is expressed in blood cells but not in brain later in chicken embryonic development. cEKLF mRNA is increased in definitive compared with primitive erythropoiesis. The conserved sequence and expression pattern of cEKLF suggests that its function is similar to its orthologues in mammals, Xenopus, and zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping