PUBLICATION

The ovalbumin serpins revisited: Perspective from the chicken genome of clade B serpin evolution in vertebrates

Authors
Benarafa, C., and Remold-O'donnell, E.
ID
ZDB-PUB-050803-10
Date
2005
Source
Proceedings of the National Academy of Sciences of the United States of America   102(32): 11367-11372 (Journal)
Registered Authors
Keywords
ov-serpin, multigene locus, maspin, MNEI, zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Chickens
  • Chromosome Mapping
  • Cluster Analysis
  • Computational Biology
  • Evolution, Molecular*
  • Gene Components
  • Genome*
  • Genomics
  • Humans
  • Models, Genetic*
  • Models, Molecular*
  • Molecular Sequence Data
  • Phylogeny*
  • Sequence Alignment
  • Serpins/chemistry
  • Serpins/genetics*
  • Zebrafish
PubMed
16055559 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Serpin superfamily proteins, most of which are serine protease inhibitors, share an unusual mechanism rooted in their conserved metastable tertiary structure. Although serpins have been identified in isolated members of archea, bacteria, and plants, a remarkable expansion is found in vertebrates. The chicken protein ovalbumin, a storage protein from egg white, lacking protease inhibitory activity, is an historical member of the superfamily and the founding member of the subgroup known as ov-serpins (ovalbumin-related serpins) or clade B serpins. In the human, ov-serpins include 13 proteins involved in the regulation of inflammation, apoptosis, angiogenesis, and embryogenesis. Here, a detailed analysis of the chicken (Gallus gallus) genome identified 10 clade B serpin genes that map to a single approximately 150-kb locus and contain the signature protein sequence of serpins and the gene structure of ov-serpins, with either seven or eight exons. Orthologues of PAI-2 (SERPINB2), MNEI (SERPINB1), PI-6 (SERPINB6), and maspin (SERPINB5) are highly conserved. Comparison with human ov-serpins identified avian-specific and mammal-specific genes. Importantly, a unique model of mammalian ov-serpin evolution is revealed from the comparative analysis of the chicken and human loci. The presence of a subset of ov-serpin genes in zebrafish (Danio rerio) gives insight into the ancestral locus. This comparative genomic study provides a valuable perspective on the evolutionary pathway for the clade B serpins, allowing the identification of genes with functions that may have been conserved since the origin of vertebrates. In addition, it suggests that "newer" serpins, such as ovalbumin, have contributed to vertebrate adaptation.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping