PUBLICATION
Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification
- Authors
- Hernandez-Lagunas, L., Choi, I.F., Kaji, T., Simpson, P., Hershey, C., Zhou, Y., Zon, L., Mercola, M., and Artinger, K.B.
- ID
- ZDB-PUB-050202-1
- Date
- 2005
- Source
- Developmental Biology 278(2): 347-357 (Journal)
- Registered Authors
- Artinger, Kristin Bruk, Choi, Irene, Hernandez, Laura, Kaji, Takao, Simpson, Pete, Zhou, Yi, Zon, Leonard I.
- Keywords
- Narrowminded; Neural crest; Rohon–Beard sensory neurons; Blimp-1
- MeSH Terms
-
- Animals
- Base Sequence
- Basic Helix-Loop-Helix Transcription Factors
- Chromosome Mapping
- DNA Primers
- DNA-Binding Proteins
- Embryo, Nonmammalian/physiology
- In Situ Hybridization
- Nerve Tissue Proteins/genetics*
- Neural Crest/physiology*
- Neurons, Afferent/cytology*
- Nuclear Proteins
- Repressor Proteins/genetics*
- Restriction Mapping
- Transcription Factors/genetics*
- Zebrafish/embryology*
- Zebrafish Proteins/genetics*
- Zinc Fingers
- PubMed
- 15680355 Full text @ Dev. Biol.
Citation
Hernandez-Lagunas, L., Choi, I.F., Kaji, T., Simpson, P., Hershey, C., Zhou, Y., Zon, L., Mercola, M., and Artinger, K.B. (2005) Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification. Developmental Biology. 278(2):347-357.
Abstract
Specification of both neural crest cells and Rohon-Beard (RB) sensory neurons involves a complex series of interactions between the neural and non-neural ectoderm. The molecular mechanisms directing this process are not well understood. The zebrafish narrowminded (nrd) mutation is unique, since it is one of two mutations in which defects are observed in both cell populations: it leads to a complete absence of RB neurons and a reduction in neural crest cells and their derivatives. Here, we show that nrd is a mutation in prdm1, a SET/zinc-finger domain transcription factor. A Morpholino-mediated depletion of prdm1 phenocopies the nrd mutation, and conversely overexpression of prdm1 mRNA rescues the nrd RB sensory neuron and neural crest phenotype. prdm1 is expressed at the border of the neural plate within the domain where neural crest cells and RB sensory neurons form. Analysis of prdm1 function by overexpression indicates that prdm1 functions to promote the cell fate specification of both neural crest cells and RB sensory neurons, most likely as a downstream effector of the BMP signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping