PUBLICATION

Expression of cyp26b1 during zebrafish early development

Authors
Zhao, Q., Dobbs-McAuliffe, B., and Linney, E.
ID
ZDB-PUB-050125-11
Date
2005
Source
Gene expression patterns : GEP   5(3): 363-369 (Journal)
Registered Authors
Dobbs-McAuliffe, Betsy, Linney, Elwood, Zhao, Qingshun
Keywords
Zebrafish; Cyp26b1; Embryo; Development; Hindbrain; Rhombomere; Retinoid signaling; Pectoral fin; Retinoic acid; Cytochrome p450
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cerebellum/enzymology
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System/biosynthesis*
  • Cytochrome P-450 Enzyme System/chemistry
  • Cytochrome P-450 Enzyme System/genetics
  • Cytochrome P-450 Enzyme System/metabolism
  • Diencephalon/enzymology
  • Gene Expression
  • In Situ Hybridization
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhombencephalon/enzymology
  • Transcription, Genetic/drug effects
  • Tretinoin/physiology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
15661642 Full text @ Gene Expr. Patterns
Abstract
We have cloned the zebrafish ortholog of the mammalian cyp26b1 gene. The predicted zebrafish cyp26b1 protein shares greater than 73% identity with mammalian homologues. cDNA transfection assays showed that like human cyp26b1, zebrafish cyp26b1 is involved in limiting the activity of retinoic acid. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of embryonic RNAs suggested that no maternal cyp26b1 message is detectable. Zygotic cyp26b1 message could be detected at 75% epiboly by RT-PCR and localized to presumptive rhombomere 3 and rhombomere 4 at the early two-somite (2S) stage (10.5 hpf: hour post fertilization) by whole mount in situ hybridization. As development proceeds expression expands anteriorly to include rhombomere 2 at the 10S stage (14hpf). By 14S (16hpf) expression in the hindbrain has also expanded posteriorly and encompasses rhombomere 2 through rhombomere 6. At later stages, 24 through 48 hpf, additional expression was found in the eyes, diencephalon, midbrain-hindbrain boundary, cerebellum, pectoral fin and the pharyngeal arch primordia.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping