PUBLICATION
Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates
- Authors
- Shike, H., Shimizu, C., Lauth, X., and Burns, J.C.
- ID
- ZDB-PUB-050727-1
- Date
- 2004
- Source
- Developmental and comparative immunology 28(7-8): 747-754 (Journal)
- Registered Authors
- Burns, Jane C.
- Keywords
- Fish; Streptococcus iniae; Infection; Hepcidin; Bacterial challenge
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Antimicrobial Cationic Peptides/genetics*
- DNA, Complementary/genetics*
- Exons/genetics*
- Hepcidins
- Humans
- Introns/genetics*
- Iron/metabolism
- Molecular Sequence Data
- Zebrafish/genetics*
- PubMed
- 15043943 Full text @ Dev. Comp. Immunol.
Citation
Shike, H., Shimizu, C., Lauth, X., and Burns, J.C. (2004) Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates. Developmental and comparative immunology. 28(7-8):747-754.
Abstract
Hepcidin is an antimicrobial peptide and iron-regulatory molecule that is conserved among vertebrates. Mutations or over-expression of the human hepcidin gene have been found in patients with hemochromatosis and refractory anemia. To further understand the function and regulation of hepcidin, animal models are needed. We sequenced cDNA, genes and upstream regions of zebrafish hepcidin and analyzed gene expression by kinetic PCR. Zebrafish hepcidin genes consist of two introns and three exons that encode a prepropeptide (91 amino acids). The amino acid sequences and gene organization were remarkably conserved between zebrafish and other species. Elevated gene expression was observed in abdominal organs, skin, and heart in fish that developed signs of infection following bacterial injection. Zebrafish may be a suitable model organism for further study of hepcidin gene regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping