PUBLICATION
Identification of 24 genes and two pseudogenes coding for olfactory receptors in Japanese loach, classified into four subfamilies: a putative evolutionary process for fish olfactory receptor genes by comprehensive phylogenetic analysis
- Authors
- Irie-Kushiyama, S., Asano-Miyoshi, M., Suda, T., Abe, K., and Emori, Y.
- ID
- ZDB-PUB-041006-10
- Date
- 2004
- Source
- Gene 325: 123-135 (Journal)
- Registered Authors
- Keywords
- Olfaction; Evolution; Fish; Multigene family
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cypriniformes/classification
- Cypriniformes/genetics*
- DNA/chemistry
- DNA/genetics
- DNA/isolation & purification
- Evolution, Molecular
- Gene Expression
- In Situ Hybridization
- Molecular Sequence Data
- Multigene Family/genetics*
- Olfactory Mucosa/metabolism
- Phylogeny*
- Pseudogenes/genetics*
- Receptors, Odorant/genetics*
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- PubMed
- 14697517 Full text @ Gene
Citation
Irie-Kushiyama, S., Asano-Miyoshi, M., Suda, T., Abe, K., and Emori, Y. (2004) Identification of 24 genes and two pseudogenes coding for olfactory receptors in Japanese loach, classified into four subfamilies: a putative evolutionary process for fish olfactory receptor genes by comprehensive phylogenetic analysis. Gene. 325:123-135.
Abstract
Twenty-four olfactory receptor (OR) genes and two pseudogenes have been identified in the genome of Japanese loach (Misgurnus anguillicaudatus). The genes were classified into four subfamilies according to the similarity of the amino acid sequences. In each subfamily, members showed high sequence similarity not only to each other but also to orthologues of other fish species. The number of members in each OR subfamily was roughly estimated to be from 3 to 10 by genomic Southern blot analysis. The genes of all four OR subfamilies were shown to express on olfactory neurons of the olfactory epithelium by in situ hybridization analysis.Two major features of fish OR genes were found by comprehensive and comparative analyses on OR genes of Japanese loach and other fish species including catfish, zebrafish and pufferfish. First, the phylogenetic tree comprising of representative subfamily members suggests the existence of several prototype genes common to the genomes of many fish species. Second, when all members of orthologous subfamilies identified in each clade of the tree are integrated, the members of a single species comprise a monophyletic group. This means that 'intraspecies' sequence homology, that is, homology among paralogous genes of the same subfamily in a species, is higher than 'interspecies' homology, that is, homology between orthologous genes of different species. This suggests that the subfamily members of a species have evolved recently. Taken together, fish OR genes have evolved from a limited number of prototype genes common to most fish species, and several genes in a subfamily have diversely evolved in each species from each prototype.
Errata / Notes
Erratum in: Gene. 2004 Mar 17;328:203-4.In Fig. 2D1, the lines which indicate the bands of MW marker overlap with the photograph. The correct Fig. 2 is reproduced here.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping