PUBLICATION
Immunocytochemical localization of neuronal and inducible nitric oxide synthase in the retina of zebrafish, Brachydanio rerio
- Authors
- Shin, D.H., Lim, H.S., Cho, S.K., Lee, H.Y., Lee, H.W., Lee, K.H., Chung, Y.H., Cho, S.S., Ik Cha, C., and Hwang, D.H.
- ID
- ZDB-PUB-001019-10
- Date
- 2000
- Source
- Neuroscience letters 292(3): 220-222 (Journal)
- Registered Authors
- Lee, Kyu-Ho
- Keywords
- neuronal nitric oxide synthase; inducible nitric oxide synthase; zebrafish; immunocytochemistry; retina
- MeSH Terms
-
- Animals
- Densitometry
- Immunohistochemistry
- Microscopy, Immunoelectron
- Nitric Oxide Synthase/metabolism*
- Nitric Oxide Synthase Type I
- Nitric Oxide Synthase Type II
- Photoreceptor Cells, Vertebrate/enzymology
- Photoreceptor Cells, Vertebrate/ultrastructure
- Retina/cytology
- Retina/enzymology*
- Retinal Ganglion Cells/cytology
- Retinal Ganglion Cells/enzymology
- Zebrafish
- PubMed
- 11018316 Full text @ Neurosci. Lett.
Citation
Shin, D.H., Lim, H.S., Cho, S.K., Lee, H.Y., Lee, H.W., Lee, K.H., Chung, Y.H., Cho, S.S., Ik Cha, C., and Hwang, D.H. (2000) Immunocytochemical localization of neuronal and inducible nitric oxide synthase in the retina of zebrafish, Brachydanio rerio. Neuroscience letters. 292(3):220-222.
Abstract
Several previous studies have revealed the distribution of neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) in the retina of various species. However, nothing has been reported on the nNOS and iNOS expression in zebrafish retina, even though it has been used as an effective model for developmental studies. In this study on nNOS and iNOS immunoreactivity (IR) in the zebrafish retina, iNOS-IR signals were detected in the ganglion cell layer (GC), the inner plexiform layer (IP), the outer plexiform layer (OP) and the photoreceptor layer (PR). nNOS-IR signals were also intensely detected in the GC, IP, OP and PR. These signals were very similar to the iNOS-IR signals, except for some minor differences in relative intensities. This could be explained by the speculation that the synthesis of nitric oxide by iNOS may represent a compensatory mechanism in the absence of nNOS. We described for the first time the distribution of nNOS and iNOS-IR cells in zebrafish retina. This could provide the basis for further study on the nNOS and iNOS properties of zebrafish retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping