PUBLICATION
Tissue-specific expression of zebrafish (Danio rerio) heat shock factor 1 mRNAS in response to heat stress
- Authors
- Råbergh, C.M.I., Airaksinen, S., Soitamo, A., Björklund, H.V., Johansson, T., Nikinmaa, M., and Sistonen, L.
- ID
- ZDB-PUB-000726-5
- Date
- 2000
- Source
- The Journal of experimental biology 203(12): 1817-1824 (Journal)
- Registered Authors
- Nikinmaa, Mikko
- Keywords
- heat shock; heat shock factor; zebrafish; Danio rerio; heat shock protein
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA-Binding Proteins/chemistry
- DNA-Binding Proteins/genetics*
- Gene Expression*
- HSP70 Heat-Shock Proteins/genetics
- Heat-Shock Response*
- Hot Temperature
- Humans
- Molecular Sequence Data
- Organ Specificity
- Phylogeny
- RNA, Messenger/analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription Factors/chemistry
- Transcription Factors/genetics*
- Transcription Factors/isolation & purification
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins*
- PubMed
- 10821739 Full text @ J. Exp. Biol.
Citation
Råbergh, C.M.I., Airaksinen, S., Soitamo, A., Björklund, H.V., Johansson, T., Nikinmaa, M., and Sistonen, L. (2000) Tissue-specific expression of zebrafish (Danio rerio) heat shock factor 1 mRNAS in response to heat stress. The Journal of experimental biology. 203(12):1817-1824.
Abstract
All organisms respond to environmental, chemical and physiological stresses by enhanced synthesis of an evolutionarily conserved family of proteins known as heat shock proteins (HSPs) or stress proteins. Certain HSPs are also expressed constitutively during cell growth and development, and they function as molecular chaperones. The transcriptional regulation of hsp genes is mediated by the heat shock transcription factor (HSF). The stress response has been studied mostly in mammalian cell lines or organisms normally maintained under constant laboratory conditions. There is much less information on the regulation of the stress response of animals, such as fish, that have to tolerate large fluctuations in environmental and internal conditions. To characterize the regulation of the heat shock response in fish, we have cloned the first heat shock transcription factor from fish, zebrafish Danio rerio. Phylogenetic analysis confirms that the isolated zebrafish HSF belongs to the HSF1 family and is therefore designated zHSF1. Analysis by reverse transcriptase polymerase chain reaction (RT-PCR) shows the presence of two zHSF1 mRNA forms that are expressed in a tissue-specific fashion upon exposure to heat stress. Both forms are expressed in gonads under all conditions; in liver and to a lesser extent in the gills, the longer splice form of zHSF1 disappears upon heat shock. We present evidence for a unique tissue-specific regulation of HSF1 upon exposure to elevated temperature.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping