CRISPR

CRISPR1-depdc5

ID

ZDB-CRISPR-190104-1

Name

CRISPR1-depdc5

Previous Names

None

Target

depdc5 (1)

Sequence

5' - TGAGGATCATGAGCAAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
udm102	depdc5

Expression

Gene expression in Wild Types + CRISPR1-depdc5

No data available

Phenotype

Phenotype resulting from CRISPR1-depdc5

No data available

Phenotype of all Fish created by or utilizing CRISPR1-depdc5

Phenotype	Fish	Conditions	Figures
whole organism decreased life span, abnormal	depdc5^{udm102/udm102} (TL)	control	Fig. 1 , Fig. 5 from Swaminathan et al., 2018
swimming occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: pentetrazol, chemical treatment by environment: vigabatrin	Fig. 6 from Swaminathan et al., 2018
musculoskeletal movement decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	control	Fig. 2 , Fig. 5 from Swaminathan et al., 2018
optic tectum neuronal action potential increased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 2 from Swaminathan et al., 2018
brain mt-cyb expression amount, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
brain gabrg2 expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
brain efnb3b expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
TOR signaling increased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: gamma-aminobutyric acid	Fig. 7 from Swaminathan et al., 2018
whole organism decreased life span, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: gamma-aminobutyric acid	Fig. 5 from Swaminathan et al., 2018
brain slc12a5b expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
whole organism depdc5 expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 1 from Swaminathan et al., 2018
brain mt-co2 expression amount, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
whole organism decreased length, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 1 from Swaminathan et al., 2018
whole organism life span, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 5 from Swaminathan et al., 2018
brain slc6a11b expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
swimming increased occurrence, exacerbated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: pentetrazol	Fig. 2 , Fig. 5 , Fig. 6 from Swaminathan et al., 2018
whole organism lethal (sensu genetics), abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 1 from Swaminathan et al., 2018
swimming occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 5 from Swaminathan et al., 2018
GABAergic neuron axon arborization decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	control	Fig. 4 , Fig. 5 from Swaminathan et al., 2018
whole organism ab1-rps6 labeling amount, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 5 from Swaminathan et al., 2018
brain slc6a11b expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
brain sema3ab expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
brain plxna2 expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
whole organism ab1-rps6 labeling increased amount, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: gamma-aminobutyric acid	Fig. 7 from Swaminathan et al., 2018
optic tectum ab1-rps6 labeling increased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 2 from Swaminathan et al., 2018
optic tectum negative regulation of neuronal action potential decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 2 from Swaminathan et al., 2018
brain mt-co2 expression increased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
swimming occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: vigabatrin	Fig. 6 from Swaminathan et al., 2018
brain mt-cyb expression increased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 7 from Swaminathan et al., 2018
GABAergic neuron axon arborization decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: gamma-aminobutyric acid	Fig. 5 from Swaminathan et al., 2018
whole organism ab1-rps6 labeling increased amount, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 2 , Fig. 5 , Fig. 7 from Swaminathan et al., 2018
swimming decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: baclofen	Fig. 6 from Swaminathan et al., 2018
musculoskeletal movement occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 5 from Swaminathan et al., 2018
TOR signaling increased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 2 , Fig. 5 , Fig. 7 from Swaminathan et al., 2018
swimming decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	standard conditions	Fig. 2 , Fig. 5 from Swaminathan et al., 2018
brain efnb3b expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
swimming occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: pentetrazol, chemical treatment by environment: sirolimus	Fig. 5 from Swaminathan et al., 2018
GABAergic neuron axon arborization decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
brain plxna2 expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
swimming occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: gamma-aminobutyric acid, chemical treatment by environment: pentetrazol	Fig. 5 from Swaminathan et al., 2018
brain slc12a5b expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
swimming decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: baclofen, chemical treatment by environment: muscimol	Fig. 6 from Swaminathan et al., 2018
brain gabrg2 expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
brain sema3ab expression decreased amount, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 7 from Swaminathan et al., 2018
TOR signaling occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: sirolimus	Fig. 5 from Swaminathan et al., 2018
swimming occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: gamma-aminobutyric acid	Fig. 5 from Swaminathan et al., 2018
musculoskeletal movement occurrence, ameliorated	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: gamma-aminobutyric acid	Fig. 5 from Swaminathan et al., 2018
swimming decreased occurrence, abnormal	depdc5^{udm102/udm102} (TL)	chemical treatment by environment: muscimol	Fig. 6 from Swaminathan et al., 2018
whole organism depdc5 expression decreased amount, abnormal	depdc5^udm102/+ (TL)	standard conditions	Fig. 1 from Swaminathan et al., 2018
whole organism ab1-rps6 labeling increased amount, abnormal	depdc5^udm102/+ (TL)	standard conditions	Fig. 2 from Swaminathan et al., 2018

Citations